Methyl jasmonate induced accumulation of kalopanaxsaponin I in Nigella sativa

Martin Scholz; Marta Lipinski; Marco Leupold; Heinrich Luftmann; Lena Harig; Rivka Ofir; Rainer Fischer; Dirk Prüfer; Kai J. Müller

doi:10.1016/j.phytochem.2009.01.018

Methyl jasmonate induced accumulation of kalopanaxsaponin I in Nigella sativa

Martin Scholz, Marta Lipinski, Marco Leupold, Heinrich Luftmann, Lena Harig, Rivka Ofir, Rainer Fischer, Dirk Prüfer, Kai J. Müller

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Hydroponically cultivated Nigella sativa L. plants treated with methyl jasmonate (MeJA) showed a twelve-fold increase in levels of the monodesmosidic triterpene saponins α-hederin and kalopanaxsaponin I (KsI) in the leaves. We will demonstrate that these two saponins accounted for approximately 10% of the dry plant matter, of which 93% was KsI and 7% α-hederin. To address the molecular basis of saponin induction by MeJA, we cloned and characterized the β-amyrin synthase gene (NsβAS1) encoding one of the key enzymes in triterpene saponin biosynthesis. As expected, NsβAS1 transcription was induced by MeJA and led to the production of β-amyrin when over-expressed in yeast.

Original language	English
Pages (from-to)	517-522
Number of pages	6
Journal	Phytochemistry
Volume	70
Issue number	4
DOIs	https://doi.org/10.1016/j.phytochem.2009.01.018
State	Published - 1 Mar 2009

Keywords

Hederins
Hydroponics
Kalopanaxsaponin I
Methyl jasmonate
Nigella sativa
Ranunculaceae
Saponin
β-Amyrin synthase

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Plant Science
Horticulture

Access to Document

10.1016/j.phytochem.2009.01.018

Cite this

@article{982bca35030b476badef061212bcd7de,

title = "Methyl jasmonate induced accumulation of kalopanaxsaponin I in Nigella sativa",

abstract = "Hydroponically cultivated Nigella sativa L. plants treated with methyl jasmonate (MeJA) showed a twelve-fold increase in levels of the monodesmosidic triterpene saponins α-hederin and kalopanaxsaponin I (KsI) in the leaves. We will demonstrate that these two saponins accounted for approximately 10% of the dry plant matter, of which 93% was KsI and 7% α-hederin. To address the molecular basis of saponin induction by MeJA, we cloned and characterized the β-amyrin synthase gene (NsβAS1) encoding one of the key enzymes in triterpene saponin biosynthesis. As expected, NsβAS1 transcription was induced by MeJA and led to the production of β-amyrin when over-expressed in yeast.",

keywords = "Hederins, Hydroponics, Kalopanaxsaponin I, Methyl jasmonate, Nigella sativa, Ranunculaceae, Saponin, β-Amyrin synthase",

author = "Martin Scholz and Marta Lipinski and Marco Leupold and Heinrich Luftmann and Lena Harig and Rivka Ofir and Rainer Fischer and Dirk Pr{\"u}fer and M{\"u}ller, {Kai J.}",

note = "Funding Information: The authors would like to thank Daniela Ahlert for superb technical assistance. This work was supported by the Bundesministerium f{\"u}r Bildung und Forschung with a Grant (FKZ 0313212) to the Institute for Biochemistry and Biotechnology of Plants.",

year = "2009",

month = mar,

day = "1",

doi = "10.1016/j.phytochem.2009.01.018",

language = "English",

volume = "70",

pages = "517--522",

journal = "Phytochemistry",

issn = "0031-9422",

publisher = "Elsevier Ltd.",

number = "4",

}

TY - JOUR

T1 - Methyl jasmonate induced accumulation of kalopanaxsaponin I in Nigella sativa

AU - Scholz, Martin

AU - Lipinski, Marta

AU - Leupold, Marco

AU - Luftmann, Heinrich

AU - Harig, Lena

AU - Ofir, Rivka

AU - Fischer, Rainer

AU - Prüfer, Dirk

AU - Müller, Kai J.

N1 - Funding Information: The authors would like to thank Daniela Ahlert for superb technical assistance. This work was supported by the Bundesministerium für Bildung und Forschung with a Grant (FKZ 0313212) to the Institute for Biochemistry and Biotechnology of Plants.

PY - 2009/3/1

Y1 - 2009/3/1

N2 - Hydroponically cultivated Nigella sativa L. plants treated with methyl jasmonate (MeJA) showed a twelve-fold increase in levels of the monodesmosidic triterpene saponins α-hederin and kalopanaxsaponin I (KsI) in the leaves. We will demonstrate that these two saponins accounted for approximately 10% of the dry plant matter, of which 93% was KsI and 7% α-hederin. To address the molecular basis of saponin induction by MeJA, we cloned and characterized the β-amyrin synthase gene (NsβAS1) encoding one of the key enzymes in triterpene saponin biosynthesis. As expected, NsβAS1 transcription was induced by MeJA and led to the production of β-amyrin when over-expressed in yeast.

AB - Hydroponically cultivated Nigella sativa L. plants treated with methyl jasmonate (MeJA) showed a twelve-fold increase in levels of the monodesmosidic triterpene saponins α-hederin and kalopanaxsaponin I (KsI) in the leaves. We will demonstrate that these two saponins accounted for approximately 10% of the dry plant matter, of which 93% was KsI and 7% α-hederin. To address the molecular basis of saponin induction by MeJA, we cloned and characterized the β-amyrin synthase gene (NsβAS1) encoding one of the key enzymes in triterpene saponin biosynthesis. As expected, NsβAS1 transcription was induced by MeJA and led to the production of β-amyrin when over-expressed in yeast.

KW - Hederins

KW - Hydroponics

KW - Kalopanaxsaponin I

KW - Methyl jasmonate

KW - Nigella sativa

KW - Ranunculaceae

KW - Saponin

KW - β-Amyrin synthase

UR - http://www.scopus.com/inward/record.url?scp=62549137403&partnerID=8YFLogxK

U2 - 10.1016/j.phytochem.2009.01.018

DO - 10.1016/j.phytochem.2009.01.018

M3 - Article

C2 - 19282005

AN - SCOPUS:62549137403

SN - 0031-9422

VL - 70

SP - 517

EP - 522

JO - Phytochemistry

JF - Phytochemistry

IS - 4

ER -

Methyl jasmonate induced accumulation of kalopanaxsaponin I in Nigella sativa

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this