Microbial shifts of faecal microbiota using enteral nutrition in vitro

Nikol Modrackova; Ivana Copova; Adam Stovicek; Marie Makovska; Dagmar Schierova; Jakub Mrazek; Monika Sabolova; Eva Vlkova; Ondrej Hradsky; Jiri Bronsky; Jiri Nevoral; Vera Neuzil-Bunesova

doi:10.1016/j.jff.2020.104330

Microbial shifts of faecal microbiota using enteral nutrition in vitro

Nikol Modrackova, Ivana Copova, Adam Stovicek, Marie Makovska, Dagmar Schierova, Jakub Mrazek, Monika Sabolova, Eva Vlkova, Ondrej Hradsky, Jiri Bronsky, Jiri Nevoral, Vera Neuzil-Bunesova

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Enteral nutrition (EN) formulas of polymeric type ordinarily have similar content of intact macronutrients but may vary in prebiotic saccharides and micronutrients. These components can play an important role in the intestinal microbiota modulation. The aim of this study was to investigate microbial changes of faecal samples after their in vitro anaerobic cultivation in four polymeric EN formulas using plate technique method, metabolite analysis, and microbiota profiling using 16S rRNA sequencing. Detected cultivable commensal groups (bifidobacteria, lactobacilli, Escherichia coli) in faecal samples of donors were able to grow in EN formulas. However, their counts varied depending on the individual donor and the type of EN formula. Similar trend was found in detected metabolites such as acetate, lactate, and butyrate. Also, taxonomic composition and diversity of original and cultivated faecal microbiota of one individual on different EN formula indicate a possible effect of the prebiotics and micronutrients to modulate gut microbiota.

Original language	English
Article number	104330
Journal	Journal of Functional Foods
Volume	77
DOIs	https://doi.org/10.1016/j.jff.2020.104330
State	Published - 1 Feb 2021
Externally published	Yes

Keywords

Cultivation
Microbiome
Polymeric enteral formulas
Prebiotics
SCFAs
Therapy

ASJC Scopus subject areas

Food Science
Medicine (miscellaneous)
Nutrition and Dietetics

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10.1016/j.jff.2020.104330

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title = "Microbial shifts of faecal microbiota using enteral nutrition in vitro",

abstract = "Enteral nutrition (EN) formulas of polymeric type ordinarily have similar content of intact macronutrients but may vary in prebiotic saccharides and micronutrients. These components can play an important role in the intestinal microbiota modulation. The aim of this study was to investigate microbial changes of faecal samples after their in vitro anaerobic cultivation in four polymeric EN formulas using plate technique method, metabolite analysis, and microbiota profiling using 16S rRNA sequencing. Detected cultivable commensal groups (bifidobacteria, lactobacilli, Escherichia coli) in faecal samples of donors were able to grow in EN formulas. However, their counts varied depending on the individual donor and the type of EN formula. Similar trend was found in detected metabolites such as acetate, lactate, and butyrate. Also, taxonomic composition and diversity of original and cultivated faecal microbiota of one individual on different EN formula indicate a possible effect of the prebiotics and micronutrients to modulate gut microbiota.",

keywords = "Cultivation, Microbiome, Polymeric enteral formulas, Prebiotics, SCFAs, Therapy",

author = "Nikol Modrackova and Ivana Copova and Adam Stovicek and Marie Makovska and Dagmar Schierova and Jakub Mrazek and Monika Sabolova and Eva Vlkova and Ondrej Hradsky and Jiri Bronsky and Jiri Nevoral and Vera Neuzil-Bunesova",

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year = "2021",

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day = "1",

doi = "10.1016/j.jff.2020.104330",

language = "English",

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T1 - Microbial shifts of faecal microbiota using enteral nutrition in vitro

AU - Modrackova, Nikol

AU - Copova, Ivana

AU - Stovicek, Adam

AU - Makovska, Marie

AU - Schierova, Dagmar

AU - Mrazek, Jakub

AU - Sabolova, Monika

AU - Vlkova, Eva

AU - Hradsky, Ondrej

AU - Bronsky, Jiri

AU - Nevoral, Jiri

AU - Neuzil-Bunesova, Vera

PY - 2021/2/1

Y1 - 2021/2/1

N2 - Enteral nutrition (EN) formulas of polymeric type ordinarily have similar content of intact macronutrients but may vary in prebiotic saccharides and micronutrients. These components can play an important role in the intestinal microbiota modulation. The aim of this study was to investigate microbial changes of faecal samples after their in vitro anaerobic cultivation in four polymeric EN formulas using plate technique method, metabolite analysis, and microbiota profiling using 16S rRNA sequencing. Detected cultivable commensal groups (bifidobacteria, lactobacilli, Escherichia coli) in faecal samples of donors were able to grow in EN formulas. However, their counts varied depending on the individual donor and the type of EN formula. Similar trend was found in detected metabolites such as acetate, lactate, and butyrate. Also, taxonomic composition and diversity of original and cultivated faecal microbiota of one individual on different EN formula indicate a possible effect of the prebiotics and micronutrients to modulate gut microbiota.

AB - Enteral nutrition (EN) formulas of polymeric type ordinarily have similar content of intact macronutrients but may vary in prebiotic saccharides and micronutrients. These components can play an important role in the intestinal microbiota modulation. The aim of this study was to investigate microbial changes of faecal samples after their in vitro anaerobic cultivation in four polymeric EN formulas using plate technique method, metabolite analysis, and microbiota profiling using 16S rRNA sequencing. Detected cultivable commensal groups (bifidobacteria, lactobacilli, Escherichia coli) in faecal samples of donors were able to grow in EN formulas. However, their counts varied depending on the individual donor and the type of EN formula. Similar trend was found in detected metabolites such as acetate, lactate, and butyrate. Also, taxonomic composition and diversity of original and cultivated faecal microbiota of one individual on different EN formula indicate a possible effect of the prebiotics and micronutrients to modulate gut microbiota.

KW - Cultivation

KW - Microbiome

KW - Polymeric enteral formulas

KW - Prebiotics

KW - SCFAs

KW - Therapy

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DO - 10.1016/j.jff.2020.104330

M3 - Article

AN - SCOPUS:85098588929

SN - 1756-4646

VL - 77

JO - Journal of Functional Foods

JF - Journal of Functional Foods

M1 - 104330

ER -

Microbial shifts of faecal microbiota using enteral nutrition in vitro

Abstract

Keywords

ASJC Scopus subject areas

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