Microfluidic arrayed lab-on-a-chip for electrochemical capacitive detection of DNA hybridization events

Hadar Ben-Yoav, Peter H. Dykstra, William E. Bentley, Reza Ghodssi

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Scopus citations


A microfluidic electrochemical lab-on-a-chip (LOC) device for DNA hybridization detection has been developed. The device comprises a 3 × 3 array of microelectrodes integrated with a dual layer microfluidic valved manipulation system that provides controlled and automated capabilities for high throughput analysis of microliter volume samples. The surface of the microelectrodes is functionalized with singlestranded DNA (ssDNA) probes which enable specific detection of complementary ssDNA targets. These targets are detected by a capacitive technique which measures dielectric variation at the microelectrode–electrolyte interface due to DNA hybridization events. A quantitative analysis of the hybridization events is carried out based on a sensing modeling that includes detailed analysis of energy storage and dissipation components. By calculating these components during hybridization events the device is able to demonstrate specific and dose response sensing characteristics. The developed microfluidic LOC for DNA hybridization detection offers a technology for real-time and label-free assessment of genetic markers outside of laboratory settings, such as at the point-of-care or in-field environmental monitoring.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages18
StatePublished - 1 Jan 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Biosensor
  • DNA hybridization sensing
  • Dielectric spectroscopy
  • Electrical equivalent model
  • Electrochemical impedance spectroscopy
  • Faradaic redox reaction
  • Lab-on-a-chip
  • Label-free detection
  • Microfluidics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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