Abstract
Nucellar embryogenesis was induced in Mangifera indica L. cv. Kurakkan, a polyembryonic salt tolerant, dwarfing rootstock. Nucellus tissue excised from 3.5 cm long fruits developed pro-embryonic callus in 19 days of inoculation on modified MS medium supplemented with 4.52μM 2,4-D, 0.05% malt extract and 13.78μM spermidine. Somatic embryogenesis exhibited high frequency (158.33 embryos). However, all the differentiated embryos proliferated on medium having low level of sucrose (4% w/v) and auxin (2.26μM 2,4-D). Most of the proembryonic calli converted into heart shaped and cotyledonary embryos by reducing temperature to 15°C. Somatic embryos were matured on modified MS medium fortified with 0.38μM ABA, 0.57μM IAA and 30.30μM PEG. Matured somatic embryos germinated (around 30%) on MS medium supplemented with 2.68μM NAA, 11.60μM kinetin and 2736.9μM glutamine.
| Original language | English |
|---|---|
| Pages (from-to) | 85-90 |
| Number of pages | 6 |
| Journal | Indian Journal of Genetics and Plant Breeding |
| Volume | 70 |
| Issue number | 1 |
| State | Published - 1 Feb 2010 |
| Externally published | Yes |
Keywords
- Abiotic stress
- Kurakkan
- Mangifera indica
- Polyembryonic
- Rootstock
- Salt tolerant
- Somatic embryogenesis
ASJC Scopus subject areas
- Genetics
- Plant Science