MicroRNA expression profiling of the human uterine cervix after term labor and delivery

Sonia S. Hassan; Roberto Romero; Beth Pineles; Adi L. Tarca; Daniel Montenegro; Offer Erez; Pooja Mittal; Juan Pedro Kusanovic; Shali Mazaki-Tovi; Jimmy Espinoza; Chia Ling Nhan-Chang; Sorin Draghici; Chong Jai Kim

doi:10.1016/j.ajog.2009.08.016

MicroRNA expression profiling of the human uterine cervix after term labor and delivery

Sonia S. Hassan, Roberto Romero, Beth Pineles, Adi L. Tarca, Daniel Montenegro, Offer Erez, Pooja Mittal, Juan Pedro Kusanovic, Shali Mazaki-Tovi, Jimmy Espinoza, Chia Ling Nhan-Chang, Sorin Draghici, Chong Jai Kim

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Objective: MicroRNAs (miRNAs) are noncoding RNAs involved in posttranscriptional regulation of target genes. The objective of this study was to determine the miRNA expression profile of the human uterine cervix after spontaneous term labor (TL). Study Design: The miRNA expression pattern of cervical tissue was characterized using microarrays. Samples were collected at term from patients with (n = 8) and without (n = 9) TL. Moderated t tests and false discovery rate correction were applied. Results were confirmed using quantitative reverse transcriptase-polymerase chain reaction. Results: A total of 226 miRNAs were expressed in human cervical tissue. miR-223, miR-34b, and miR-34c were overexpressed in cervical tissue of patients with TL compared to those without. Quantitative reverse transcriptase-polymerase chain reaction assays confirmed these findings (miR-223 [fold change {FC} = 5.7], miR-34b [FC = 4.5], miR-34c [FC = 6.2]; P < .001). Conclusion: This is the first report of miRNA expression in the human uterine cervix in pregnancy. Cervical remodeling after TL and delivery was associated with changes in miR-223, miR-34b, and miR-34c.

Original language	English
Pages (from-to)	80.e1-80.e8
Journal	American Journal of Obstetrics and Gynecology
Volume	202
Issue number	1
DOIs	https://doi.org/10.1016/j.ajog.2009.08.016
State	Published - 1 Jan 2010
Externally published	Yes

Keywords

cervical dilation
cervical remodeling
cervix
labor
microRNA
microarray
parturition

ASJC Scopus subject areas

Obstetrics and Gynecology

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10.1016/j.ajog.2009.08.016

Cite this

Hassan, S. S., Romero, R., Pineles, B., Tarca, A. L., Montenegro, D., Erez, O., Mittal, P., Kusanovic, J. P., Mazaki-Tovi, S., Espinoza, J., Nhan-Chang, C. L., Draghici, S., & Kim, C. J. (2010). MicroRNA expression profiling of the human uterine cervix after term labor and delivery. American Journal of Obstetrics and Gynecology, 202(1), 80.e1-80.e8. https://doi.org/10.1016/j.ajog.2009.08.016

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title = "MicroRNA expression profiling of the human uterine cervix after term labor and delivery",

abstract = "Objective: MicroRNAs (miRNAs) are noncoding RNAs involved in posttranscriptional regulation of target genes. The objective of this study was to determine the miRNA expression profile of the human uterine cervix after spontaneous term labor (TL). Study Design: The miRNA expression pattern of cervical tissue was characterized using microarrays. Samples were collected at term from patients with (n = 8) and without (n = 9) TL. Moderated t tests and false discovery rate correction were applied. Results were confirmed using quantitative reverse transcriptase-polymerase chain reaction. Results: A total of 226 miRNAs were expressed in human cervical tissue. miR-223, miR-34b, and miR-34c were overexpressed in cervical tissue of patients with TL compared to those without. Quantitative reverse transcriptase-polymerase chain reaction assays confirmed these findings (miR-223 [fold change {FC} = 5.7], miR-34b [FC = 4.5], miR-34c [FC = 6.2]; P < .001). Conclusion: This is the first report of miRNA expression in the human uterine cervix in pregnancy. Cervical remodeling after TL and delivery was associated with changes in miR-223, miR-34b, and miR-34c.",

keywords = "cervical dilation, cervical remodeling, cervix, labor, microRNA, microarray, parturition",

author = "Hassan, {Sonia S.} and Roberto Romero and Beth Pineles and Tarca, {Adi L.} and Daniel Montenegro and Offer Erez and Pooja Mittal and Kusanovic, {Juan Pedro} and Shali Mazaki-Tovi and Jimmy Espinoza and Nhan-Chang, {Chia Ling} and Sorin Draghici and Kim, {Chong Jai}",

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doi = "10.1016/j.ajog.2009.08.016",

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Hassan, SS, Romero, R, Pineles, B, Tarca, AL, Montenegro, D, Erez, O, Mittal, P, Kusanovic, JP, Mazaki-Tovi, S, Espinoza, J, Nhan-Chang, CL, Draghici, S & Kim, CJ 2010, 'MicroRNA expression profiling of the human uterine cervix after term labor and delivery', American Journal of Obstetrics and Gynecology, vol. 202, no. 1, pp. 80.e1-80.e8. https://doi.org/10.1016/j.ajog.2009.08.016

TY - JOUR

T1 - MicroRNA expression profiling of the human uterine cervix after term labor and delivery

AU - Hassan, Sonia S.

AU - Romero, Roberto

AU - Pineles, Beth

AU - Tarca, Adi L.

AU - Montenegro, Daniel

AU - Erez, Offer

AU - Mittal, Pooja

AU - Kusanovic, Juan Pedro

AU - Mazaki-Tovi, Shali

AU - Espinoza, Jimmy

AU - Nhan-Chang, Chia Ling

AU - Draghici, Sorin

AU - Kim, Chong Jai

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Objective: MicroRNAs (miRNAs) are noncoding RNAs involved in posttranscriptional regulation of target genes. The objective of this study was to determine the miRNA expression profile of the human uterine cervix after spontaneous term labor (TL). Study Design: The miRNA expression pattern of cervical tissue was characterized using microarrays. Samples were collected at term from patients with (n = 8) and without (n = 9) TL. Moderated t tests and false discovery rate correction were applied. Results were confirmed using quantitative reverse transcriptase-polymerase chain reaction. Results: A total of 226 miRNAs were expressed in human cervical tissue. miR-223, miR-34b, and miR-34c were overexpressed in cervical tissue of patients with TL compared to those without. Quantitative reverse transcriptase-polymerase chain reaction assays confirmed these findings (miR-223 [fold change {FC} = 5.7], miR-34b [FC = 4.5], miR-34c [FC = 6.2]; P < .001). Conclusion: This is the first report of miRNA expression in the human uterine cervix in pregnancy. Cervical remodeling after TL and delivery was associated with changes in miR-223, miR-34b, and miR-34c.

AB - Objective: MicroRNAs (miRNAs) are noncoding RNAs involved in posttranscriptional regulation of target genes. The objective of this study was to determine the miRNA expression profile of the human uterine cervix after spontaneous term labor (TL). Study Design: The miRNA expression pattern of cervical tissue was characterized using microarrays. Samples were collected at term from patients with (n = 8) and without (n = 9) TL. Moderated t tests and false discovery rate correction were applied. Results were confirmed using quantitative reverse transcriptase-polymerase chain reaction. Results: A total of 226 miRNAs were expressed in human cervical tissue. miR-223, miR-34b, and miR-34c were overexpressed in cervical tissue of patients with TL compared to those without. Quantitative reverse transcriptase-polymerase chain reaction assays confirmed these findings (miR-223 [fold change {FC} = 5.7], miR-34b [FC = 4.5], miR-34c [FC = 6.2]; P < .001). Conclusion: This is the first report of miRNA expression in the human uterine cervix in pregnancy. Cervical remodeling after TL and delivery was associated with changes in miR-223, miR-34b, and miR-34c.

KW - cervical dilation

KW - cervical remodeling

KW - cervix

KW - labor

KW - microRNA

KW - microarray

KW - parturition

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U2 - 10.1016/j.ajog.2009.08.016

DO - 10.1016/j.ajog.2009.08.016

M3 - Article

C2 - 19889381

AN - SCOPUS:72449173767

SN - 0002-9378

VL - 202

SP - 80.e1-80.e8

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

IS - 1

ER -

MicroRNA expression profiling of the human uterine cervix after term labor and delivery

Abstract

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