TY - JOUR
T1 - Microspot Sensing Based on Surface-Enhanced Fluorescence from Nanosculptured Thin Films
AU - Karabchevsky, Alina
AU - Khare, Chinmay
AU - Rauschenbach, Bernd
AU - Abdulhalim, Ibrahim
N1 - Funding Information:
The authors are grateful to Dr. Christian Patzig and Professor Akhlesh Lakhtakia for the useful discussions during the early stages of this research. Chinmay Khare would like to acknowledge the Graduate school BuildMoNa at University Leipzig in Germany [within the German Excellence Initiative of the Deutsche Forschungsgemeinschaft (DFG)], for its financial support. This work is partially supported by the Singapore National Research Foundation CREATE program “Nanomaterials for Energy and Water Management.”
PY - 2012/6/21
Y1 - 2012/6/21
N2 - Nanosculptured thin films (STF) are prepared by the oblique angle deposition technique and take different forms of nano columnar structures. Varieties of STFs were investigated to find the optimum structure for biosensing based on the surface enhanced fluorescence. A comparative study was carried out with STFs containing the nanocolumnar structures that differ in their shape, height (h), and tilt angle with respect to the surface (α), thickness (d), and arrangement. The greatest enhancement of the fluorescent signal was found for Ag-based STFs on Si(100), giving an enhancement factor of ×71, where h=400nm, d=75nm, and α=23° relative to Ag closed film using fluorescent dye Rhodamine 123. We immobilized the fluorescent receptor to the thiol self-assembly monolayer on Ag-based STF and Ag dense film to demonstrate the applications of STFs for specific biosensing. Upon excitation of the fluorophore by an Hg light source, a CCD camera with controlled exposure time would detect the pattern of fluorescent receptor Anti-Rabbit IgG on the surfaces. A specially designed optical fiber housing attached to the microscope allowed quantitative measurement of the fluorescence spectrum on a microspot parallel to the image grab.
AB - Nanosculptured thin films (STF) are prepared by the oblique angle deposition technique and take different forms of nano columnar structures. Varieties of STFs were investigated to find the optimum structure for biosensing based on the surface enhanced fluorescence. A comparative study was carried out with STFs containing the nanocolumnar structures that differ in their shape, height (h), and tilt angle with respect to the surface (α), thickness (d), and arrangement. The greatest enhancement of the fluorescent signal was found for Ag-based STFs on Si(100), giving an enhancement factor of ×71, where h=400nm, d=75nm, and α=23° relative to Ag closed film using fluorescent dye Rhodamine 123. We immobilized the fluorescent receptor to the thiol self-assembly monolayer on Ag-based STF and Ag dense film to demonstrate the applications of STFs for specific biosensing. Upon excitation of the fluorophore by an Hg light source, a CCD camera with controlled exposure time would detect the pattern of fluorescent receptor Anti-Rabbit IgG on the surfaces. A specially designed optical fiber housing attached to the microscope allowed quantitative measurement of the fluorescence spectrum on a microspot parallel to the image grab.
KW - fluorescence spectroscopy
KW - optical biosensing
KW - sculptured thin films
KW - surface enhanced fluorescence
UR - http://www.scopus.com/inward/record.url?scp=84864135828&partnerID=8YFLogxK
U2 - 10.1117/1.JNP.6.061508
DO - 10.1117/1.JNP.6.061508
M3 - Article
AN - SCOPUS:84864135828
SN - 1934-2608
VL - 6
JO - Journal of Nanophotonics
JF - Journal of Nanophotonics
IS - 1
M1 - 061508
ER -