Microtubules, motor proteins, and anomalous mean squared displacements

Hanna Salman, Yotam Gil, Rony Granek, Michael Elbaum

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

We report an experimental study of the dynamics of particles driven along biopolymer tracks by active protein motors. We use extracts of Xenopus laevis eggs, which support the formation of cytoskeletal networks (microtubules and actin filaments) in vitro and provide all the biochemical factors needed for particle motion. 3 μm polystyrene beads adsorb motor proteins non-specifically and serve as the transport substrate. We observe an enhanced diffusive motion with time scaling of the mean squared displacement as t3/2. At the shortest times, the data indicate a crossover to a subdiffusive or saturated regime, due to intermittent adhesion of the motor proteins to the microtubules. The time interval over which this behavior was observed varied widely. On inhibiting one or the other family of the microtubule-associated motor proteins, the interval on which such saturation was observed increased, yet the t3/2 behavior at longer times was unaffected.

Original languageEnglish
Pages (from-to)389-397
Number of pages9
JournalChemical Physics
Volume284
Issue number1-2
DOIs
StatePublished - 1 Nov 2002
Externally publishedYes

ASJC Scopus subject areas

  • General Physics and Astronomy
  • Physical and Theoretical Chemistry

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