Mimicking Horseradish Peroxidase and NADH Peroxidase by Heterogeneous Cu2+-Modified Graphene Oxide Nanoparticles

Shan Wang; Rémi Cazelles; Wei Ching Liao; Margarita Vázquez-González; Amani Zoabi; Raed Abu-Reziq; Itamar Willner

doi:10.1021/acs.nanolett.7b00093

Mimicking Horseradish Peroxidase and NADH Peroxidase by Heterogeneous Cu²⁺-Modified Graphene Oxide Nanoparticles

Shan Wang, Rémi Cazelles, Wei Ching Liao, Margarita Vázquez-González, Amani Zoabi, Raed Abu-Reziq, Itamar Willner

Research output: Contribution to journal › Article › peer-review

201 Scopus citations

Abstract

Cu²⁺-ion-modified graphene oxide nanoparticles, Cu²⁺-GO NPs, act as a heterogeneous catalyst mimicking functions of horseradish peroxidase, HRP, and of NADH peroxidase. The Cu²⁺-GO NPs catalyze the oxidation of dopamine to aminochrome by H₂O₂ and catalyze the generation of chemiluminescence in the presence of luminol and H₂O₂. The Cu²⁺-GO NPs provide an active material for the chemiluminescence detection of H₂O₂ and allow the probing of the activity of H₂O₂-generating oxidases and the detection of their substrates. This is exemplified with detecting glucose by the aerobic oxidation of glucose by glucose oxidase and the Cu²⁺-GO NP-stimulated chemiluminescence intensity generated by the H₂O₂ product. Similarly, the Cu²⁺-GO NPs catalyze the H₂O₂ oxidation of NADH to the biologically active NAD⁺ cofactor.

Original language	English
Pages (from-to)	2043-2048
Number of pages	6
Journal	Nano Letters
Volume	17
Issue number	3
DOIs	https://doi.org/10.1021/acs.nanolett.7b00093
State	Published - 8 Mar 2017
Externally published	Yes

Keywords

Catalysis
biocatalysis
chemiluminescence
dopamine
nanotechnology

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

Access to Document

10.1021/acs.nanolett.7b00093

Cite this

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title = "Mimicking Horseradish Peroxidase and NADH Peroxidase by Heterogeneous Cu2+-Modified Graphene Oxide Nanoparticles",

abstract = "Cu2+-ion-modified graphene oxide nanoparticles, Cu2+-GO NPs, act as a heterogeneous catalyst mimicking functions of horseradish peroxidase, HRP, and of NADH peroxidase. The Cu2+-GO NPs catalyze the oxidation of dopamine to aminochrome by H2O2 and catalyze the generation of chemiluminescence in the presence of luminol and H2O2. The Cu2+-GO NPs provide an active material for the chemiluminescence detection of H2O2 and allow the probing of the activity of H2O2-generating oxidases and the detection of their substrates. This is exemplified with detecting glucose by the aerobic oxidation of glucose by glucose oxidase and the Cu2+-GO NP-stimulated chemiluminescence intensity generated by the H2O2 product. Similarly, the Cu2+-GO NPs catalyze the H2O2 oxidation of NADH to the biologically active NAD+ cofactor.",

keywords = "Catalysis, biocatalysis, chemiluminescence, dopamine, nanotechnology",

author = "Shan Wang and R{\'e}mi Cazelles and Liao, {Wei Ching} and Margarita V{\'a}zquez-Gonz{\'a}lez and Amani Zoabi and Raed Abu-Reziq and Itamar Willner",

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doi = "10.1021/acs.nanolett.7b00093",

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T1 - Mimicking Horseradish Peroxidase and NADH Peroxidase by Heterogeneous Cu2+-Modified Graphene Oxide Nanoparticles

AU - Wang, Shan

AU - Cazelles, Rémi

AU - Liao, Wei Ching

AU - Vázquez-González, Margarita

AU - Zoabi, Amani

AU - Abu-Reziq, Raed

AU - Willner, Itamar

PY - 2017/3/8

Y1 - 2017/3/8

N2 - Cu2+-ion-modified graphene oxide nanoparticles, Cu2+-GO NPs, act as a heterogeneous catalyst mimicking functions of horseradish peroxidase, HRP, and of NADH peroxidase. The Cu2+-GO NPs catalyze the oxidation of dopamine to aminochrome by H2O2 and catalyze the generation of chemiluminescence in the presence of luminol and H2O2. The Cu2+-GO NPs provide an active material for the chemiluminescence detection of H2O2 and allow the probing of the activity of H2O2-generating oxidases and the detection of their substrates. This is exemplified with detecting glucose by the aerobic oxidation of glucose by glucose oxidase and the Cu2+-GO NP-stimulated chemiluminescence intensity generated by the H2O2 product. Similarly, the Cu2+-GO NPs catalyze the H2O2 oxidation of NADH to the biologically active NAD+ cofactor.

AB - Cu2+-ion-modified graphene oxide nanoparticles, Cu2+-GO NPs, act as a heterogeneous catalyst mimicking functions of horseradish peroxidase, HRP, and of NADH peroxidase. The Cu2+-GO NPs catalyze the oxidation of dopamine to aminochrome by H2O2 and catalyze the generation of chemiluminescence in the presence of luminol and H2O2. The Cu2+-GO NPs provide an active material for the chemiluminescence detection of H2O2 and allow the probing of the activity of H2O2-generating oxidases and the detection of their substrates. This is exemplified with detecting glucose by the aerobic oxidation of glucose by glucose oxidase and the Cu2+-GO NP-stimulated chemiluminescence intensity generated by the H2O2 product. Similarly, the Cu2+-GO NPs catalyze the H2O2 oxidation of NADH to the biologically active NAD+ cofactor.

KW - Catalysis

KW - biocatalysis

KW - chemiluminescence

KW - dopamine

KW - nanotechnology

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