TY - JOUR
T1 - Mitochondria isolated from lipid droplets of white adipose tissue reveal functional differences based on lipid droplet size
AU - Brownstein, Alexandra J.
AU - Veliova, Michaela
AU - Acin-Perez, Rebeca
AU - Villalobos, Frankie
AU - Petcherski, Anton
AU - Tombolato, Alberto
AU - Liesa, Marc
AU - Shirihai, Orian S.
N1 - Publisher Copyright:
© 2023 Brownstein et al.
PY - 2024/2/1
Y1 - 2024/2/1
N2 - Recent studies in brown adipose tissue (BAT) described a unique subpopulation of mitochondria bound to lipid droplets (LDs), which were termed PeriDroplet Mitochondria (PDM). PDM can be isolated from BAT by differential centrifugation and salt washes. Contrary to BAT, this approach has so far not led to the successful isolation of PDM from white adipose tissue (WAT). Here, we de-veloped a method to isolate PDM from WAT with high yield and purity by an optimized proteolytic treatment that preserves the respiratory function of mitochondria. Using this approach, we show that, contrary to BAT, WAT PDM have lower respiratory and ATP synthesis capacities compared with WAT cytoplasmic mitochondria (CM). Furthermore, by isolating PDM from LDs of different sizes, we found a negative correlation between LD size and the respiratory capacity of their PDM in WAT. Thus, our new isolation method reveals tissue-specific characteristics of PDM and establishes the existence of heterogeneity in PDM function determined by LD size.
AB - Recent studies in brown adipose tissue (BAT) described a unique subpopulation of mitochondria bound to lipid droplets (LDs), which were termed PeriDroplet Mitochondria (PDM). PDM can be isolated from BAT by differential centrifugation and salt washes. Contrary to BAT, this approach has so far not led to the successful isolation of PDM from white adipose tissue (WAT). Here, we de-veloped a method to isolate PDM from WAT with high yield and purity by an optimized proteolytic treatment that preserves the respiratory function of mitochondria. Using this approach, we show that, contrary to BAT, WAT PDM have lower respiratory and ATP synthesis capacities compared with WAT cytoplasmic mitochondria (CM). Furthermore, by isolating PDM from LDs of different sizes, we found a negative correlation between LD size and the respiratory capacity of their PDM in WAT. Thus, our new isolation method reveals tissue-specific characteristics of PDM and establishes the existence of heterogeneity in PDM function determined by LD size.
UR - http://www.scopus.com/inward/record.url?scp=85179018259&partnerID=8YFLogxK
U2 - 10.26508/lsa.202301934
DO - 10.26508/lsa.202301934
M3 - Article
C2 - 38056907
AN - SCOPUS:85179018259
SN - 2575-1077
VL - 7
JO - Life Science Alliance
JF - Life Science Alliance
IS - 2
M1 - e202301934
ER -