Mitochondrial manganese superoxide dismutase mRNA expression in human chorioamniotic membranes and its association with labor, inflammation, and infection

Nandor Gabor Than, Roberto Romero, Adi L. Tarca, Sorin Draghici, Offer Erez, Tinnakorn Chaiworapongsa, Yeon Mee Kim, Sun Kwon Kim, Edi Vaisbuch, Gerard Tromp

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47 Scopus citations

Abstract

Objective.Human parturition is characterized by the activation of genes involved in acute inflammatory responses in the fetal membranes. Manganese superoxide dismutase (Mn SOD) is a mitochondrial enzyme that scavenges reactive oxygen species (ROS). Mn SOD is up-regulated in sites of inflammation and has an important role in the down-regulation of acute inflammatory processes. Therefore, the aim of this study was to determine the differences in Mn SOD mRNA expression in the fetal membranes in patients with term and preterm labor (PTL) as well as in acute chorioamnionitis. Study design.Fetal membranes were obtained from patients in the following groups: (1) term not in labor (n=29); (2) term in labor (n=29); 3 spontaneous PTL with intact mebranes (n=16); (4) PTL with histological chorioamnionitis (n=12); (5) preterm prelabor rupture of the membranes (PPROM; n=17); and (6) PPROM with histological chorioamnionitis (n=21). Mn SOD mRNA expression in the membranes was determined by quantitative real-time reverse transcription-polymerase chain reaction. Results. (1) Mn SOD mRNA expression was higher in the fetal membranes of patients at term in labor than those not in labor (2.4-fold; p=0.02); (2) the amount of Mn SOD mRNA in the fetal membranes was higher in PTL than in term labor or in PPROM (7.2-fold, p=0.03; 3.2-fold, p=0.03, respectively); (3) Mn SOD mRNA expression was higher when histological chorioamnionitis was present both among patients with PPROM (3.8-fold, p=0.02) and with PTL (5.4-fold, p=0.02) than in patients with these conditions without histological chorioamnionitis; 4 expression of Mn SOD mRNA was higher in PTL with chorioamnionitis than in PPROM with chorioamnionitis (4.3-fold, p=0.03). Conclusion.The increase in Mn SOD mRNA expression by fetal membranes in term labor and in histological chorioamnionitis in PTL and PPROM suggests that the fetus deploys anti-oxidant mechanisms to constrain the inflammatory processes in the chorioamniotic membranes.

Original languageEnglish
Pages (from-to)1000-1013
Number of pages14
JournalJournal of Maternal-Fetal and Neonatal Medicine
Volume22
Issue number11
DOIs
StatePublished - 1 Nov 2009
Externally publishedYes

Keywords

  • Fetal gender
  • Gene expression
  • Preterm delivery
  • Preterm labor
  • Preterm prelabor rupture of the membranes
  • Reactive oxygen species
  • Scavenger

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

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