Mitochondrial responses to organelle-specific drug delivering nanoparticles composed of polypeptide and peptide complexes

Carleigh Charles, Ifat Cohen-Erez, Blake Kazaoka, Olga Melnikov, David E. Stein, Richard Sensenig, Hanna Rapaport, Zulfiya Orynbayeva

Research output: Contribution to journalArticlepeer-review

Abstract

Aims: The mechanistic study of the drug carrier-target interactions of mitochondria-unique nanoparticles composed of polypeptide-peptide complexes (mPoP-NPs). Materials & methods: The isolated organelles were employed to address the direct effects of mPoP-NPs on dynamic structure and functional wellbeing of mitochondria. Mitochondria morphology, respiration, membrane potential, reactive oxygen species generation, were examined by confocal microscopy, flow cytometry and oxygraphy. Lonidamine-encapsulated formulation was assessed to evaluate the drug delivery capacity of the naive nanoparticles. Results: The mPoP-NPs do not alter mitochondria structure and performance upon docking to organelles, while successfully delivering drug that causes organelle dysfunction. Conclusion: The study gives insight into interactions of mPoP-NPs with mitochondria and provides substantial support for consideration of designed nanoparticles as biocompatible and efficient mitochondria-targeted platforms.

Original languageEnglish
Pages (from-to)2917-2932
Number of pages16
JournalNanomedicine
Volume15
Issue number30
DOIs
StatePublished - 1 Dec 2020

Keywords

  • OxPhos
  • TPP
  • cancer
  • lonidamine
  • membrane potential
  • mitochondria
  • nanoparticles

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science (all)

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