@article{712b7a9fac61446e8a953e379adeb391,
title = "Model-assisted identification of metabolic engineering strategies for Jatropha curcas lipid pathways",
abstract = "Efficient approaches to increase plant lipid production are necessary to meet current industrial demands for this important resource. While Jatropha curcas cell culture can be used for in vitro lipid production, scaling up the system for industrial applications requires an understanding of how growth conditions affect lipid metabolism and yield. Here we present a bottom-up metabolic reconstruction of J. curcas supported with labeling experiments and biomass characterization under three growth conditions. We show that the metabolic model can accurately predict growth and distribution of fluxes in cell cultures and use these findings to pinpoint energy expenditures that affect lipid biosynthesis and metabolism. In addition, by using constraint-based modeling approaches we identify network reactions whose joint manipulation optimizes lipid production. The proposed model and computational analyses provide a stepping stone for future rational optimization of other agronomically relevant traits in J. curcas.",
keywords = "Jatropha curcas, cell cultures, constrained-based analysis, lipid biosynthesis and metabolism, metabolic engineering, metabolic reconstruction",
author = "Correa, {Sandra M.} and Saleh Alseekh and Luc{\'i}a Atehort{\'u}a and Yariv Brotman and Rigoberto R{\'i}os-Estepa and Fernie, {Alisdair R.} and Zoran Nikoloski",
note = "Funding Information: Thanks to the Biotechnology Laboratory-Universidad de Antioquia and the Max Planck Institute of Molecular Plant Physiology for the use of their infrastructure. Thanks to ?nne Michaelis, Gudrun Wolter, Andr? Scheffel, Arun Sampathkumar, Rainer H?fgen, Franziska Br?ckner, Stephanie Arrivault, Manuela Guenther, Patrick Giavalisco, Laise Rosado, Elmien Heyneke, Jos? Vallarino, Fayezeh Arabi, Karin K?hl, Regina Wendenburg and Ina Krahnert for their assistance during different parts of the experimental work. We are grateful to Carlos Palacio (Colombiana de Biocombustibles) for supplying the plant material, to N?stor Giraldo and Luis Velasquez for their assistance with some aspects of the experimental work and to Michael Scheunemann for his help with some technical issues in the modeling procedure. The work was financially supported by Fondo Nacional de Financiamiento para la Ciencia, la Tecnolog?a y la Innovaci?n Francisco Jos? de Caldas-Colciencias (grant no. 617/2013) and the DAAD (grant no. 57214225). Funding Information: Thanks to the Biotechnology Laboratory‐Universidad de Antioquia and the Max Planck Institute of Molecular Plant Physiology for the use of their infrastructure. Thanks to {\"A}nne Michaelis, Gudrun Wolter, Andr{\'e} Scheffel, Arun Sampathkumar, Rainer H{\"o}fgen, Franziska Br{\"u}ckner, Stephanie Arrivault, Manuela Guenther, Patrick Giavalisco, Laise Rosado, Elmien Heyneke, Jos{\'e} Vallarino, Fayezeh Arabi, Karin K{\"o}hl, Regina Wendenburg and Ina Krahnert for their assistance during different parts of the experimental work. We are grateful to Carlos Palacio (Colombiana de Biocombustibles) for supplying the plant material, to N{\'e}stor Giraldo and Luis Velasquez for their assistance with some aspects of the experimental work and to Michael Scheunemann for his help with some technical issues in the modeling procedure. The work was financially supported by Fondo Nacional de Financiamiento para la Ciencia, la Tecnolog{\'i}a y la Innovaci{\'o}n Francisco Jos{\'e} de Caldas‐Colciencias (grant no. 617/2013) and the DAAD (grant no. 57214225). Publisher Copyright: {\textcopyright} 2020 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd",
year = "2020",
month = sep,
day = "1",
doi = "10.1111/tpj.14906",
language = "English",
volume = "104",
pages = "76--95",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "1",
}