Modeling the Combining Site of the Human Asialoglycoprotein Receptor

Sharona Khier, Sandra Tolchinsky, Gerardo Z. Lederkremer, Boaz Shaanan

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

A model for the carbohydrate recognition domain (CRD) and combining site of the human asialoglycoprotein (ASGP) receptor has been computed on the basis of the close sequence homology with the mannose‐binding lectin (MBP), whose three‐dimensional structure in complex with a ligand has been determined by crystallographic methods (Weis, W.I.; Drickamer, K.; Hendrickson, W.A. Nature 1992, 360: 127). Within the limitations of modeling methods, the model is compatible with data on ligand binding of the family C‐type lectins, of which the MBP and the ASGP receptor are members. The model derived can serve as a guide for designing site‐directed mutagenesis experiments in order to further elucidate the origins of specificity of the ASGP receptor toward galactose. In particular, the model focuses attention on the possible role of position 207 (MBP sequence numbering) in promoting galactose binding.

Original languageEnglish
Pages (from-to)177-184
Number of pages8
JournalIsrael Journal of Chemistry
Volume34
Issue number2
DOIs
StatePublished - 1 Jan 1994
Externally publishedYes

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