TY - JOUR
T1 - Modulation of gut microbiota by spent coffee grounds attenuates diet-induced metabolic syndrome in rats
AU - Bhandarkar, Nikhil S.
AU - Mouatt, Peter
AU - Goncalves, Priscila
AU - Thomas, Torsten
AU - Brown, Lindsay
AU - Panchal, Sunil K.
N1 - Funding Information:
The authors thank CCP Café at the Toowoomba campus of the University of Southern Queensland for donating spent coffee grounds. We thank Brian Bynon, School of Veterinary Sciences, The University of Queensland, Gatton Campus, for plasma biochemical analyses and the Analytical Research Laboratory, Southern Cross University, Lismore, NSW for the facilities required for phytochemical analyses. This work was supported by funding received from the University of Southern Queensland Research and Innovation Division.
Funding Information:
The authors thank CCP Café at the Toowoomba campus of the University of Southern Queensland for donating spent coffee grounds. We thank Brian Bynon, School of Veterinary Sciences, The University of Queensland, Gatton Campus, for plasma biochemical analyses and the Analytical Research Laboratory, Southern Cross University, Lismore, NSW for the facilities required for phytochemical analyses. This work was supported by funding received from the University of Southern Queensland Research and Innovation Division.
Publisher Copyright:
© 2020 Federation of American Societies for Experimental Biology
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Coffee brewing produces spent coffee grounds as waste; few studies have investigated the health benefits of these grounds. This study investigated responses to spent coffee grounds in a diet-induced rat model of metabolic syndrome. Male Wistar rats aged 8-9 weeks were fed either corn starch-rich diet or high-carbohydrate, high-fat diet for 16 weeks, which were supplemented with 5% spent coffee grounds during the last 8 weeks. Rats fed non-supplemented diets were used as controls. High-carbohydrate, high-fat diet-fed rats developed metabolic syndrome including abdominal obesity, impaired glucose tolerance, dyslipidemia, and cardiovascular and liver damage. Body weight, abdominal fat, total body fat mass, systolic blood pressure, and concentrations of plasma triglycerides and non-esterified fatty acids were reduced by spent coffee grounds along with improved glucose tolerance and structure and function of heart and liver. Spent coffee grounds increased the diversity of the gut microbiota and decreased the ratio of Firmicutes to Bacteroidetes. Changes in gut microbiota correlated with the reduction in obesity and improvement in glucose tolerance and systolic blood pressure. These findings indicate that intervention with spent coffee grounds may be useful for managing obesity and metabolic syndrome by altering the gut microbiota, thus increasing the value of this food waste.
AB - Coffee brewing produces spent coffee grounds as waste; few studies have investigated the health benefits of these grounds. This study investigated responses to spent coffee grounds in a diet-induced rat model of metabolic syndrome. Male Wistar rats aged 8-9 weeks were fed either corn starch-rich diet or high-carbohydrate, high-fat diet for 16 weeks, which were supplemented with 5% spent coffee grounds during the last 8 weeks. Rats fed non-supplemented diets were used as controls. High-carbohydrate, high-fat diet-fed rats developed metabolic syndrome including abdominal obesity, impaired glucose tolerance, dyslipidemia, and cardiovascular and liver damage. Body weight, abdominal fat, total body fat mass, systolic blood pressure, and concentrations of plasma triglycerides and non-esterified fatty acids were reduced by spent coffee grounds along with improved glucose tolerance and structure and function of heart and liver. Spent coffee grounds increased the diversity of the gut microbiota and decreased the ratio of Firmicutes to Bacteroidetes. Changes in gut microbiota correlated with the reduction in obesity and improvement in glucose tolerance and systolic blood pressure. These findings indicate that intervention with spent coffee grounds may be useful for managing obesity and metabolic syndrome by altering the gut microbiota, thus increasing the value of this food waste.
KW - gut microbiota
KW - metabolic syndrome
KW - obesity
KW - rats
KW - spent coffee grounds
UR - http://www.scopus.com/inward/record.url?scp=85079460253&partnerID=8YFLogxK
U2 - 10.1096/fj.201902416RR
DO - 10.1096/fj.201902416RR
M3 - Article
C2 - 32039529
AN - SCOPUS:85079460253
SN - 0892-6638
VL - 34
SP - 4783
EP - 4797
JO - FASEB Journal
JF - FASEB Journal
IS - 3
ER -