Modulation of progesterone metabolizing enzyme in lymphocytes during development and by endocrine manipulation

Y. Weinstein; A. S. Fuks

doi:10.1016/b978-0-08-024940-7.50016-0

Modulation of progesterone metabolizing enzyme in lymphocytes during development and by endocrine manipulation

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Abstract

20α-Hydroxysteroid dehydrogenase (20α-SDH), an enzyme which reduces progesterone to 20α-dihydroprogesterone, was found to be associated with T lymphocytes. 20α-SDH activity was present in spleen cells bearing θ antigen in a T lymphocyteenriched population of spleen cells (nonadherent to nylon wool) and in thymocytes. T cell mitogens (PHA and Con A), but not the B cell mitogen LPS, induced high levels of enzymic activity 48 h after their addition to spleen cell cultures. Therefore high levels of enzymic activity seems characteristic of T cells stimulated to proliferate. The level of 20α-SDH activity in lymphocytes was age dependent; its activity in thymocytes, spleen cells and lymph node lymphocytes was 3 to 5 times higher at 4 weeks than at 8 and 16 weeks. Progesterone (5.0 × 10^-7M), but not 20α-dihydroprogesterone (10^-6M), was found to inhibit thymocyte proliferation after exposure to mitogens. Thus, 20α-SDH may protect the embryonic thymocytes against high concentrations of progesterone. The activity of 20α-SDH was measured in thymus and spleen cells from NZB, NZW and (NZB × NZW)f₁ ( B W) mice. There were no sex differences in the 20α-SDH activity of thymic and splenic lymphocytes in NZB and NZW mice. Orchidectomy, but not ovariectomy, caused a significant decrease in thymocyte 20α-SDH activity. Injection of 75 μg testosterone propionate on the 13th day of gestation depressed 20α-SDH activity in thymic and spleen cells of young (1- to 2-month-old) mice. The 20α-SDH activity of fetal liver lymphocytes from B W mice was twice as high as in either parent strain. No 20α-SDH activity was found in fetal liver lymphocytes taken from BALB C, SJL or C57BL 6 mice. The use of 20α-SDH as a marker for pre-T and T lymphocytes permits demonstration of the effect of sex hormones on the immune system. This enzymic marker is also a probe for the abnormal behavior of pre-T and T lymphocytes in mouse strains (NZB, B W) susceptible to autoimmune diseases.

Original language	English
Pages (from-to)	209-221
Number of pages	13
Journal	Advances in the Biosciences
Volume	25
Issue number	C
DOIs	https://doi.org/10.1016/b978-0-08-024940-7.50016-0
State	Published - 1 Jan 1980
Externally published	Yes

Keywords

20α-hydroxysteroid/dehydrogenase
NZB mice
T lymphocytes
autoimmune disease
progesterone
testosterone
thymocytes

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10.1016/b978-0-08-024940-7.50016-0

Cite this

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title = "Modulation of progesterone metabolizing enzyme in lymphocytes during development and by endocrine manipulation",

abstract = "20α-Hydroxysteroid dehydrogenase (20α-SDH), an enzyme which reduces progesterone to 20α-dihydroprogesterone, was found to be associated with T lymphocytes. 20α-SDH activity was present in spleen cells bearing θ antigen in a T lymphocyteenriched population of spleen cells (nonadherent to nylon wool) and in thymocytes. T cell mitogens (PHA and Con A), but not the B cell mitogen LPS, induced high levels of enzymic activity 48 h after their addition to spleen cell cultures. Therefore high levels of enzymic activity seems characteristic of T cells stimulated to proliferate. The level of 20α-SDH activity in lymphocytes was age dependent; its activity in thymocytes, spleen cells and lymph node lymphocytes was 3 to 5 times higher at 4 weeks than at 8 and 16 weeks. Progesterone (5.0 × 10-7M), but not 20α-dihydroprogesterone (10-6M), was found to inhibit thymocyte proliferation after exposure to mitogens. Thus, 20α-SDH may protect the embryonic thymocytes against high concentrations of progesterone. The activity of 20α-SDH was measured in thymus and spleen cells from NZB, NZW and (NZB × NZW)f1 ( B W) mice. There were no sex differences in the 20α-SDH activity of thymic and splenic lymphocytes in NZB and NZW mice. Orchidectomy, but not ovariectomy, caused a significant decrease in thymocyte 20α-SDH activity. Injection of 75 μg testosterone propionate on the 13th day of gestation depressed 20α-SDH activity in thymic and spleen cells of young (1- to 2-month-old) mice. The 20α-SDH activity of fetal liver lymphocytes from B W mice was twice as high as in either parent strain. No 20α-SDH activity was found in fetal liver lymphocytes taken from BALB C, SJL or C57BL 6 mice. The use of 20α-SDH as a marker for pre-T and T lymphocytes permits demonstration of the effect of sex hormones on the immune system. This enzymic marker is also a probe for the abnormal behavior of pre-T and T lymphocytes in mouse strains (NZB, B W) susceptible to autoimmune diseases.",

keywords = "20α-hydroxysteroid/dehydrogenase, NZB mice, T lymphocytes, autoimmune disease, progesterone, testosterone, thymocytes",

author = "Y. Weinstein and Fuks, {A. S.}",

year = "1980",

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doi = "10.1016/b978-0-08-024940-7.50016-0",

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AU - Weinstein, Y.

AU - Fuks, A. S.

PY - 1980/1/1

Y1 - 1980/1/1

N2 - 20α-Hydroxysteroid dehydrogenase (20α-SDH), an enzyme which reduces progesterone to 20α-dihydroprogesterone, was found to be associated with T lymphocytes. 20α-SDH activity was present in spleen cells bearing θ antigen in a T lymphocyteenriched population of spleen cells (nonadherent to nylon wool) and in thymocytes. T cell mitogens (PHA and Con A), but not the B cell mitogen LPS, induced high levels of enzymic activity 48 h after their addition to spleen cell cultures. Therefore high levels of enzymic activity seems characteristic of T cells stimulated to proliferate. The level of 20α-SDH activity in lymphocytes was age dependent; its activity in thymocytes, spleen cells and lymph node lymphocytes was 3 to 5 times higher at 4 weeks than at 8 and 16 weeks. Progesterone (5.0 × 10-7M), but not 20α-dihydroprogesterone (10-6M), was found to inhibit thymocyte proliferation after exposure to mitogens. Thus, 20α-SDH may protect the embryonic thymocytes against high concentrations of progesterone. The activity of 20α-SDH was measured in thymus and spleen cells from NZB, NZW and (NZB × NZW)f1 ( B W) mice. There were no sex differences in the 20α-SDH activity of thymic and splenic lymphocytes in NZB and NZW mice. Orchidectomy, but not ovariectomy, caused a significant decrease in thymocyte 20α-SDH activity. Injection of 75 μg testosterone propionate on the 13th day of gestation depressed 20α-SDH activity in thymic and spleen cells of young (1- to 2-month-old) mice. The 20α-SDH activity of fetal liver lymphocytes from B W mice was twice as high as in either parent strain. No 20α-SDH activity was found in fetal liver lymphocytes taken from BALB C, SJL or C57BL 6 mice. The use of 20α-SDH as a marker for pre-T and T lymphocytes permits demonstration of the effect of sex hormones on the immune system. This enzymic marker is also a probe for the abnormal behavior of pre-T and T lymphocytes in mouse strains (NZB, B W) susceptible to autoimmune diseases.

AB - 20α-Hydroxysteroid dehydrogenase (20α-SDH), an enzyme which reduces progesterone to 20α-dihydroprogesterone, was found to be associated with T lymphocytes. 20α-SDH activity was present in spleen cells bearing θ antigen in a T lymphocyteenriched population of spleen cells (nonadherent to nylon wool) and in thymocytes. T cell mitogens (PHA and Con A), but not the B cell mitogen LPS, induced high levels of enzymic activity 48 h after their addition to spleen cell cultures. Therefore high levels of enzymic activity seems characteristic of T cells stimulated to proliferate. The level of 20α-SDH activity in lymphocytes was age dependent; its activity in thymocytes, spleen cells and lymph node lymphocytes was 3 to 5 times higher at 4 weeks than at 8 and 16 weeks. Progesterone (5.0 × 10-7M), but not 20α-dihydroprogesterone (10-6M), was found to inhibit thymocyte proliferation after exposure to mitogens. Thus, 20α-SDH may protect the embryonic thymocytes against high concentrations of progesterone. The activity of 20α-SDH was measured in thymus and spleen cells from NZB, NZW and (NZB × NZW)f1 ( B W) mice. There were no sex differences in the 20α-SDH activity of thymic and splenic lymphocytes in NZB and NZW mice. Orchidectomy, but not ovariectomy, caused a significant decrease in thymocyte 20α-SDH activity. Injection of 75 μg testosterone propionate on the 13th day of gestation depressed 20α-SDH activity in thymic and spleen cells of young (1- to 2-month-old) mice. The 20α-SDH activity of fetal liver lymphocytes from B W mice was twice as high as in either parent strain. No 20α-SDH activity was found in fetal liver lymphocytes taken from BALB C, SJL or C57BL 6 mice. The use of 20α-SDH as a marker for pre-T and T lymphocytes permits demonstration of the effect of sex hormones on the immune system. This enzymic marker is also a probe for the abnormal behavior of pre-T and T lymphocytes in mouse strains (NZB, B W) susceptible to autoimmune diseases.

KW - 20α-hydroxysteroid/dehydrogenase

KW - NZB mice

KW - T lymphocytes

KW - autoimmune disease

KW - progesterone

KW - testosterone

KW - thymocytes

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M3 - Article

AN - SCOPUS:49149146703

SN - 0065-3446

VL - 25

SP - 209

EP - 221

JO - Advances in the Biosciences

JF - Advances in the Biosciences

IS - C

ER -

Modulation of progesterone metabolizing enzyme in lymphocytes during development and by endocrine manipulation

Abstract

Keywords

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