TY - JOUR
T1 - Modulation of protein kinase C and Ca2+ lipid‐independent protein kinase in lymphoma induced by moloney murine leukemia virus in BALB/c mice
AU - Wolfson, M.
AU - Aboud, M.
AU - Ofir, R.
AU - Weinstein, Y.
AU - Segal, S.
PY - 1986/1/1
Y1 - 1986/1/1
N2 - We investigated the possible role of protein kinase C (PKC) in the progression of Moloney murine leukemia virus (Mo‐MuLV)‐induced lymphoma in BALB/c mice. Mice injected with Mo‐MuLV on the first day after birth developed lymphoma within 1 1/2 3 months. The development of lymphoma was characterized by a gradual increase in the number of spleen cells. However, no analogous changes could be detected in the thymuses of these mice, although cells of both organs were found to be virus producers as early as 3‐4 weeks after inoculation. PKC activity, which was assayed in extracts of spleen and thymus cells, declined gradually during the development of lymphoma. Concomitantly with this decline, a progressive appearance of Ca2+/ lipid‐independent protein kinase activity was observed. TPA treatment of intact cells from normal mice reduced the level of soluble PKC activity, while inducing Ca2+/lipid‐independent phosphorylation. By contrast, TPA had no effect on these enzymatic activities in cells derived from leukemic mice. Spleen enlargement caused by injection of a non‐leukemogenic inflammatory agent such as mineral oil was ineffective in this respect, suggesting that the PKC‐Ca2+ /lipid‐independent protein kinase modulation is associated with the virally induced leukemogenesis.
AB - We investigated the possible role of protein kinase C (PKC) in the progression of Moloney murine leukemia virus (Mo‐MuLV)‐induced lymphoma in BALB/c mice. Mice injected with Mo‐MuLV on the first day after birth developed lymphoma within 1 1/2 3 months. The development of lymphoma was characterized by a gradual increase in the number of spleen cells. However, no analogous changes could be detected in the thymuses of these mice, although cells of both organs were found to be virus producers as early as 3‐4 weeks after inoculation. PKC activity, which was assayed in extracts of spleen and thymus cells, declined gradually during the development of lymphoma. Concomitantly with this decline, a progressive appearance of Ca2+/ lipid‐independent protein kinase activity was observed. TPA treatment of intact cells from normal mice reduced the level of soluble PKC activity, while inducing Ca2+/lipid‐independent phosphorylation. By contrast, TPA had no effect on these enzymatic activities in cells derived from leukemic mice. Spleen enlargement caused by injection of a non‐leukemogenic inflammatory agent such as mineral oil was ineffective in this respect, suggesting that the PKC‐Ca2+ /lipid‐independent protein kinase modulation is associated with the virally induced leukemogenesis.
UR - http://www.scopus.com/inward/record.url?scp=0022600351&partnerID=8YFLogxK
U2 - 10.1002/ijc.2910370418
DO - 10.1002/ijc.2910370418
M3 - Article
AN - SCOPUS:0022600351
SN - 0020-7136
VL - 37
SP - 589
EP - 593
JO - International Journal of Cancer
JF - International Journal of Cancer
IS - 4
ER -