Molecular approaches to biochemical purification: The COP9 complex paradigm

DA Chamovitz, XW Deng

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Light signals perceived from the photoreceptors are transduced into the nucleus to regulate gene expression and development. The nuclear-localized COP9 protein complex has a central role in modulating light-regulated development, and may have a role in regulating development in general in all organisms. We have employed a never molecular approach to traditional biochemical methodologies in order to purify the COP9 complex. As the COP9 gene has been cloned without knowing the biochemical activity of its protein product, the COP9 complex was purified from cauliflower by assaying for the presence of COP9 by immune-blot analysis. This approach should be applicable to studying other proteins which have been identified through genetic and molecular means, but whose activities are not known.
Original languageEnglish
Title of host publicationCELLULAR INTEGRATION OF SIGNALLING PATHWAYS IN PLANT DEVELOPMENT
EditorsF LoSchiavo, NV Raikhel
Pages83-91
Volume104
StatePublished - 1998
Externally publishedYes

Publication series

NameNATO ADVANCED SCIENCE INSTITUTES SERIES, SERIES H, CELL BIOLOGY

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