Molecular Approaches to Biochemical Purification: The COP9 Complex Paradigm

Daniel A. Chamovitz; Xing-Wang Deng

doi:10.1007/978-3-642-72117-5_8

Molecular Approaches to Biochemical Purification: The COP9 Complex Paradigm

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Light signals perceived from the photoreceptors are transduced into the nucleus to regulate gene expression and development. The nuclear-localized COP9 protein complex has a central role in modulating light-regulated development, and may have a role in regulating development in general in all organisms. We have employed a novel molecular approach to traditional biochemical methodologies in order to purify the COP9 complex. As the COP9 gene has been cloned without knowing the biochemical activity of its protein product, the COP9 complex was purified from cauliflower by assaying for the presence of COP9 by immuno-blot analysis. This approach should be applicable to studying other proteins which have been identified through genetic and molecular means, but whose activities are not known.

Original language	English
Title of host publication	Cellular Integration of Signalling Pathways in Plant Development
Editors	Fiorella Lo Schiavo, Robert L. Last, Giorgio Morelli, Natasha V. Raikhel
Place of Publication	Berlin, Heidelberg
Publisher	Springer Berlin Heidelberg
Pages	83-91
Number of pages	9
Volume	104
ISBN (Electronic)	978-3-642-72117-5
ISBN (Print)	978-3-642-72119-9
DOIs	https://doi.org/10.1007/978-3-642-72117-5_8
State	Published - 1998
Externally published	Yes

Publication series

Name	NATO ASI Series
Publisher	Springer Berlin
Volume	104
ISSN (Print)	1010-8793

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10.1007/978-3-642-72117-5_8

Cite this

Chamovitz, D. A., & Deng, X.-W. (1998). Molecular Approaches to Biochemical Purification: The COP9 Complex Paradigm. In F. Lo Schiavo, R. L. Last, G. Morelli, & N. V. Raikhel (Eds.), Cellular Integration of Signalling Pathways in Plant Development (Vol. 104, pp. 83-91). (NATO ASI Series; Vol. 104). Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-642-72117-5_8

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title = "Molecular Approaches to Biochemical Purification: The COP9 Complex Paradigm",

abstract = "Light signals perceived from the photoreceptors are transduced into the nucleus to regulate gene expression and development. The nuclear-localized COP9 protein complex has a central role in modulating light-regulated development, and may have a role in regulating development in general in all organisms. We have employed a novel molecular approach to traditional biochemical methodologies in order to purify the COP9 complex. As the COP9 gene has been cloned without knowing the biochemical activity of its protein product, the COP9 complex was purified from cauliflower by assaying for the presence of COP9 by immuno-blot analysis. This approach should be applicable to studying other proteins which have been identified through genetic and molecular means, but whose activities are not known.",

author = "Chamovitz, {Daniel A.} and Xing-Wang Deng",

year = "1998",

doi = "10.1007/978-3-642-72117-5_8",

language = "English",

isbn = "978-3-642-72119-9",

volume = "104",

series = "NATO ASI Series",

publisher = "Springer Berlin Heidelberg",

pages = "83--91",

editor = "{Lo Schiavo}, Fiorella and Last, {Robert L.} and Giorgio Morelli and Raikhel, {Natasha V.}",

booktitle = "Cellular Integration of Signalling Pathways in Plant Development",

}

Chamovitz, DA & Deng, X-W 1998, Molecular Approaches to Biochemical Purification: The COP9 Complex Paradigm. in F Lo Schiavo, RL Last, G Morelli & NV Raikhel (eds), Cellular Integration of Signalling Pathways in Plant Development. vol. 104, NATO ASI Series, vol. 104, Springer Berlin Heidelberg, Berlin, Heidelberg, pp. 83-91. https://doi.org/10.1007/978-3-642-72117-5_8

Molecular Approaches to Biochemical Purification: The COP9 Complex Paradigm. / Chamovitz, Daniel A.; Deng, Xing-Wang.
Cellular Integration of Signalling Pathways in Plant Development. ed. / Fiorella Lo Schiavo; Robert L. Last; Giorgio Morelli; Natasha V. Raikhel. Vol. 104 Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. p. 83-91 (NATO ASI Series; Vol. 104).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Molecular Approaches to Biochemical Purification: The COP9 Complex Paradigm

AU - Chamovitz, Daniel A.

AU - Deng, Xing-Wang

PY - 1998

Y1 - 1998

N2 - Light signals perceived from the photoreceptors are transduced into the nucleus to regulate gene expression and development. The nuclear-localized COP9 protein complex has a central role in modulating light-regulated development, and may have a role in regulating development in general in all organisms. We have employed a novel molecular approach to traditional biochemical methodologies in order to purify the COP9 complex. As the COP9 gene has been cloned without knowing the biochemical activity of its protein product, the COP9 complex was purified from cauliflower by assaying for the presence of COP9 by immuno-blot analysis. This approach should be applicable to studying other proteins which have been identified through genetic and molecular means, but whose activities are not known.

AB - Light signals perceived from the photoreceptors are transduced into the nucleus to regulate gene expression and development. The nuclear-localized COP9 protein complex has a central role in modulating light-regulated development, and may have a role in regulating development in general in all organisms. We have employed a novel molecular approach to traditional biochemical methodologies in order to purify the COP9 complex. As the COP9 gene has been cloned without knowing the biochemical activity of its protein product, the COP9 complex was purified from cauliflower by assaying for the presence of COP9 by immuno-blot analysis. This approach should be applicable to studying other proteins which have been identified through genetic and molecular means, but whose activities are not known.

U2 - 10.1007/978-3-642-72117-5_8

DO - 10.1007/978-3-642-72117-5_8

M3 - Conference contribution

SN - 978-3-642-72119-9

VL - 104

T3 - NATO ASI Series

SP - 83

EP - 91

BT - Cellular Integration of Signalling Pathways in Plant Development

A2 - Lo Schiavo, Fiorella

A2 - Last, Robert L.

A2 - Morelli, Giorgio

A2 - Raikhel, Natasha V.

PB - Springer Berlin Heidelberg

CY - Berlin, Heidelberg

ER -

Molecular Approaches to Biochemical Purification: The COP9 Complex Paradigm

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