Light signals perceived from the photoreceptors are transduced into the nucleus to regulate gene expression and development. The nuclear-localized COP9 protein complex has a central role in modulating light-regulated development, and may have a role in regulating development in general in all organisms. We have employed a never molecular approach to traditional biochemical methodologies in order to purify the COP9 complex. As the COP9 gene has been cloned without knowing the biochemical activity of its protein product, the COP9 complex was purified from cauliflower by assaying for the presence of COP9 by immune-blot analysis. This approach should be applicable to studying other proteins which have been identified through genetic and molecular means, but whose activities are not known.
|Title of host publication||CELLULAR INTEGRATION OF SIGNALLING PATHWAYS IN PLANT DEVELOPMENT|
|Editors||F LoSchiavo, NV Raikhel|
|State||Published - 1998|
|Name||NATO ADVANCED SCIENCE INSTITUTES SERIES, SERIES H, CELL BIOLOGY|