TY - JOUR
T1 - Molecular characterization of a common fragile site (FRA7H) on human chromosome 7 by the cloning of a simian virus 40 integration site
AU - Mishmar, Dan
AU - Rahat, Ayelet
AU - Scherer, Stephen W.
AU - Nyakatura, Gerald
AU - Hinzmann, Bernd
AU - Kohwi, Yoshinori
AU - Mandel-Gutfroind, Yael
AU - Lee, Jeffrey R.
AU - Drescher, Bernd
AU - Sas, Dean E.
AU - Margalit, Hanah
AU - Platzer, Mattias
AU - Weiss, Aryeh
AU - Tsui, Lap Chee
AU - Rosenthal, André
AU - Kerem, Batsheva
PY - 1998/7/7
Y1 - 1998/7/7
N2 - Common fragile sites are chromosomal loci prone to breakage and rearrangement, hypothesized to provide targets for foreign DNA integration. We cloned a simian virus 40 integration site and showed by fluorescent in situ hybridization analysis that the integration event had occurred within a common aphidicolin-induced fragile site on human chromosome 7, FRA7H. A region of 161 kb spanning FRA7H was defined and sequenced. Several regions with a potential unusual DNA structure, including high-flexibility, low- stability, and non-B-DNA-forming sequences were identified in this region. We performed a similar analysis on the published FRA3B sequence and the putative partial FRA7G, which also revealed an impressive cluster of regions with high flexibility and low stability. Thus, these unusual DNA characteristics are possibly intrinsic properties of common fragile sites that may affect their replication and condensation as well as organization, and may lead to fragility.
AB - Common fragile sites are chromosomal loci prone to breakage and rearrangement, hypothesized to provide targets for foreign DNA integration. We cloned a simian virus 40 integration site and showed by fluorescent in situ hybridization analysis that the integration event had occurred within a common aphidicolin-induced fragile site on human chromosome 7, FRA7H. A region of 161 kb spanning FRA7H was defined and sequenced. Several regions with a potential unusual DNA structure, including high-flexibility, low- stability, and non-B-DNA-forming sequences were identified in this region. We performed a similar analysis on the published FRA3B sequence and the putative partial FRA7G, which also revealed an impressive cluster of regions with high flexibility and low stability. Thus, these unusual DNA characteristics are possibly intrinsic properties of common fragile sites that may affect their replication and condensation as well as organization, and may lead to fragility.
UR - http://www.scopus.com/inward/record.url?scp=13144283613&partnerID=8YFLogxK
U2 - 10.1073/pnas.95.14.8141
DO - 10.1073/pnas.95.14.8141
M3 - Article
C2 - 9653154
AN - SCOPUS:13144283613
SN - 0027-8424
VL - 95
SP - 8141
EP - 8146
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 14
ER -