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Molecular identification of aedes triseriatus and aedes hendersoni by a novel duplex polymerase chain reaction assay

  • Robert Wilson
  • , Robin Harrison
  • , Michael Riles
  • , Gideon Wasserberg
  • , Brian D. Byrd

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Aedes triseriatus is the principal vector of La Crosse virus (LACv), which is the most common cause of pediatric arboviral encephalitis in North America. Here we report a novel species-specific polymerase chain reaction (PCR) assay that differentially identifies Ae. triseriatus and Ae. hendersoni. Because these 2 sibling species differ in their abilities to transmit LACv, accurate identification is critical for surveillance, research, and control programs. This duplex assay can detect the presence of both species in a single PCR reaction and is therefore simpler and faster than previously reported methods.

Original languageEnglish
Pages (from-to)79-82
Number of pages4
JournalJournal of the American Mosquito Control Association
Volume30
Issue number2
DOIs
StatePublished - 1 Jan 2014
Externally publishedYes

Keywords

  • Aedes hendersoni
  • Aedes triseriatus
  • La Crosse encephalitis
  • Ochlerotatus hendersoni
  • Ochlerotatus triseriatus

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Public Health, Environmental and Occupational Health
  • Insect Science

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