Molybdenum-based diazotrophy in a Sphagnum peatland in northern Minnesota

Melissa J. Warren, Xueju Lin, John C. Gaby, Cecilia B. Kretz, Max Kolton, Peter L. Morton, Jennifer Pett-Ridge, David J. Weston, Christopher W. Schadt, Joel E. Kostka, Jennifer B. Glass

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Microbial N2 fixation (diazotrophy) represents an important nitrogen source to oligotrophic peatland ecosystems, which are important sinks for atmospheric CO2 and are susceptible to the changing climate. The objectives of this study were (i) to determine the active microbial group and type of nitrogenase mediating diazotrophy in an ombrotrophic Sphagnum-dominated peat bog (the S1 peat bog, Marcell Experimental Forest, Minnesota, USA); and (ii) to determine the effect of environmental parameters (light, O2, CO2, and CH4) on potential rates of diazotrophy measured by acetylene (C2H2) reduction and 15N2 incorporation. A molecular analysis of metabolically active microbial communities suggested that diazotrophy in surface peat was primarily mediated by Alphaproteobacteria (Bradyrhizobiaceae and Beijerinckiaceae). Despite higher concentrations of dissolved vanadium ([V] 11 nM) than molybdenum ([Mo] 3 nM) in surface peat, a combination of metagenomic, amplicon sequencing, and activity measurements indicated that Mo-containing nitrogenases dominate over the V-containing form. Acetylene reduction was only detected in surface peat exposed to light, with the highest rates observed in peat collected from hollows with the highest water contents. Incorporation of 15N2 was suppressed 90% by O2 and 55% by C2H2 and was unaffected by CH4 and CO2 amendments. These results suggest that peatland diazotrophy is mediated by a combination of C2H2- sensitive and C2H2-insensitive microbes that are more active at low concentrations of O2 and show similar activity at high and low concentrations of CH4.

Original languageEnglish
Article numbere01174-17
JournalApplied and Environmental Microbiology
Volume83
Issue number17
DOIs
StatePublished - 1 Sep 2017
Externally publishedYes

Keywords

  • Acetylene
  • Alphaproteobacteria
  • Diazotrophy
  • Methanotrophs
  • Molybdenum
  • Nitrogen cycle enzymes
  • Nitrogen fixation
  • Peatland
  • Sphagnum
  • Vanadium

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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