Monitoring of blood oxygenation in brain by resonance Raman spectroscopy

Nadezda A. Brazhe, Kirsten Thomsen, Micael Lønstrup, Alexey R. Brazhe, Evelina I. Nikelshparg, Georgy V. Maksimov, Martin Lauritzen, Olga Sosnovtseva

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Blood oxygenation in cerebral vessels is an essential parameter to evaluate brain function and to investigate the coupling between local blood flow and neuronal activity. We apply resonance Raman spectroscopy in vivo to study hemoglobin oxygenation in cortex vessels of anesthetized ventilated mice. We demonstrate that the pairs of Raman peaks at 1355 and1375 cm−1 (symmetric vibrations of pyrrol half-rings in the heme molecule), 1552 and 1585 cm−1 and 1602 and 1638 cm−1 (vibrations of methine bridges in heme molecule) are reliable markers for quantitative estimation of the relative amount of oxyhemoglobin in venules, arterioles, and capillaries. in vivo measurements of blood oxygenation in the cortex of mice ventilated with inspiratory gas mixtures containing different amounts of oxygen—normoxia, hyperoxia and hypoxia—validate the proposed approach. Our method allows to visualize blood saturation with O2 in different microvascular networks.

Original languageEnglish
Article numbere201700311
JournalJournal of Biophotonics
Issue number6
StatePublished - 1 Jun 2018
Externally publishedYes


  • blood oxygenation
  • brain
  • hemoglobin
  • resonance Raman spectroscopy
  • vessels

ASJC Scopus subject areas

  • General Chemistry
  • General Materials Science
  • General Biochemistry, Genetics and Molecular Biology
  • General Engineering
  • General Physics and Astronomy


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