Monomer Dynamics in Double- and Single-Stranded DNA Polymers

Roman Shusterman, Sergey Alon, Tatyana Gavrinyov, Oleg Krichevsky

Research output: Contribution to journalArticlepeer-review

126 Scopus citations

Abstract

We present the first measurements of the kinetics of random motion of individual monomers within large polymer coils. We use double- and single-stranded DNA (dsDNA and ssDNA) as models of semiflexible and flexible polymers, respectively. Fluorescence fluctuations of DNA fragments labeled specifically at a single position reveal the time dependence of the DNA monomer’s mean-square displacement ⟨r2(t)⟩. The monomer motions within dsDNA and ssDNA coils are characterized by two qualitatively different kinetic regimes: close to ⟨r2(t)⟩∝t2/3 for ssDNA and ⟨r2(t)⟩∝√t for dsDNA. While the kinetic behavior of ssDNA is consistent with the generally accepted Zimm theory of polymer dynamics, the kinetic behavior of dsDNA monomers is in good agreement with the Rouse model.
Original languageEnglish
Article number048303
Pages (from-to)483031-483034
Number of pages4
JournalPhysical Review Letters
Volume92
Issue number4
DOIs
StatePublished - 30 Jan 2004

ASJC Scopus subject areas

  • General Physics and Astronomy

Fingerprint

Dive into the research topics of 'Monomer Dynamics in Double- and Single-Stranded DNA Polymers'. Together they form a unique fingerprint.

Cite this