Mosquito larvicidal activity of transgenic Anabaena PCC 7120 expressing toxin genes from Bacillus thuringiensis subsp. israelensis

Vadim Khasdan, Eitan Ben-Dov, Robert Manasherob, Sammy Boussiba, Arieh Zaritsky

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Genes encoding the mosquito larvicidal toxins Cry4Aa, Cry11Aa, Cyt1Aa and the regulatory P20 from Bacillus thuringiensis subsp. israelensis were introduced into the nitrogen-fixing, filamentous cyanobacterium Anabaena PCC 7120 for expression under control of two strong promoters PpsbA and PA1. The clone pRVE4-ADRC displayed toxicity against fourth-instar larvae of Aedes aegypti, the highest ever achieved in cyanobacteria. It was about 2.5-fold more toxic than the respective clone without cyt1Aa [Wu et al., Appl. Environ. Microbiol. 63 (1997) 4971-4975]. Cyt1Aa synergized the combination of Crys by about five-fold. Consistently, the lethal times exerted by pRVE4-ADRC were also reduced (it killed exposed larvae more quickly). This clone may become a useful biological control agent which reduces the probability of resistance development in the target organisms [Wirth et al., Proc. Natl. Acad. Sci. USA 94 (1997) 10536-10540].

Original languageEnglish
Pages (from-to)189-195
Number of pages7
JournalFEMS Microbiology Letters
Volume227
Issue number2
DOIs
StatePublished - 24 Oct 2003

Keywords

  • Bacillus thuringiensis subsp. israelensis
  • Mosquitocidal Anabaena
  • δ-Endotoxin

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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