TY - JOUR
T1 - Mosquito larvicidal potential of Gossypium hirsutum (Bt cotton) Leaves extracts against Aedes aegypti and Anopheles stephensi larvae
AU - Patil, Chandrashekhar D.
AU - Borase, Hemant P.
AU - Salunkhe, Rahul B.
AU - Suryawanshi, Rahul K.
AU - Narkhade, Chandrakant P.
AU - Salunke, Bipinchandra K.
AU - Patil, Satish V.
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Background: We aimed to extract the ingredients from leaves of Gossypium hirsutum (Bt cotton) using different solvents and evaluate for potential use to control different larval stages of mosquito species, Aedes aegypti and Anopheles stephensi. Methods: Qualitative and quantitative estimation of ingredients from Go. hirsutum (Bt) plant extract was carried out and their inhibitory action against mosquito larvae was determined using mosquito larvicidal assay. Results: LC50 values of water, ethanol, ethyl acetate and hexane extracts for Ae. aegypti were 211.73±21.49, 241.64±19.92, 358.07±32.43, 401.03±36.19 and 232.56±26.00, 298.54±21.78, 366.50±30.59, 387.19±31.82 for 4th instar of An. stephensi, respectively. The water extract displayed lowest LC50 value followed by ethanol, ethyl acetate and hexane. Owing to the comparatively better activity of water extract, its efficacy was further evaluated for mosquito larvicidal activity, which exhibited LC50 values of 133.95±12.79, 167.65±11.34 against 2nd and 3rd instars of Ae. aegypti and 145.48±11.76, 188.10±12.92 against 2nd and 3rd instars of An. stephensi, respectively. Crude protein from the water extract was precipitated using acetone and tested against 2nd, 3rd and 4th instars of Ae. aegypti and An. stephensi. It revealed further decrease in LC50 values as 105.72±25.84, 138.23±23.18, 126.19±25.65, 134.04±04 and 137.88±17.59, 154.25±16.98 for 2nd, 3rd and 4th instars of Ae. aegypti and An. stephensi, respectively. Conclusion: Leaves extracts of Go. hirsutum (Bt) is potential mosquito larvicide and can be used as a potent alternative to chemical insecticides in integrated pest management.
AB - Background: We aimed to extract the ingredients from leaves of Gossypium hirsutum (Bt cotton) using different solvents and evaluate for potential use to control different larval stages of mosquito species, Aedes aegypti and Anopheles stephensi. Methods: Qualitative and quantitative estimation of ingredients from Go. hirsutum (Bt) plant extract was carried out and their inhibitory action against mosquito larvae was determined using mosquito larvicidal assay. Results: LC50 values of water, ethanol, ethyl acetate and hexane extracts for Ae. aegypti were 211.73±21.49, 241.64±19.92, 358.07±32.43, 401.03±36.19 and 232.56±26.00, 298.54±21.78, 366.50±30.59, 387.19±31.82 for 4th instar of An. stephensi, respectively. The water extract displayed lowest LC50 value followed by ethanol, ethyl acetate and hexane. Owing to the comparatively better activity of water extract, its efficacy was further evaluated for mosquito larvicidal activity, which exhibited LC50 values of 133.95±12.79, 167.65±11.34 against 2nd and 3rd instars of Ae. aegypti and 145.48±11.76, 188.10±12.92 against 2nd and 3rd instars of An. stephensi, respectively. Crude protein from the water extract was precipitated using acetone and tested against 2nd, 3rd and 4th instars of Ae. aegypti and An. stephensi. It revealed further decrease in LC50 values as 105.72±25.84, 138.23±23.18, 126.19±25.65, 134.04±04 and 137.88±17.59, 154.25±16.98 for 2nd, 3rd and 4th instars of Ae. aegypti and An. stephensi, respectively. Conclusion: Leaves extracts of Go. hirsutum (Bt) is potential mosquito larvicide and can be used as a potent alternative to chemical insecticides in integrated pest management.
KW - Aaegypt
KW - Cotton
KW - Leaves extract
KW - Mosquito
KW - Toxicity
UR - http://www.scopus.com/inward/record.url?scp=84890866140&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:84890866140
SN - 2322-1984
VL - 8
SP - 91
EP - 101
JO - Journal of Arthropod-Borne Diseases
JF - Journal of Arthropod-Borne Diseases
IS - 1
ER -
