TY - UNPB
T1 - mRNA secondary structure stability regulates bacterial translation insulation and re-initiation
AU - Chemla, Yonatan
AU - Peeri, Michael
AU - Heltberg, Mathias Luidor
AU - Eichler, Jerry
AU - Jensen, Mogens HØgh
AU - Tuller, Tamir
AU - Alfonta, Lital
PY - 2020/2/10
Y1 - 2020/2/10
N2 - In bacteria, translation re-initiation is crucial for synthesizing proteins encoded by genes that are organized into operons. The mechanisms regulating translation re-initiation remain, however, poorly understood. We now describe the ribosome termination structure (RTS), a conserved and stable mRNA secondary structure precisely localized downstream of stop codons, which serves as the main factor governing re-initiation efficiency in a synthetic Escherichia coli operon. We further report that in 95% of 128 analyzed bacterial genomes representing all phyla, this structure is selectively depleted when re-initiation is advantageous yet selectively enriched so as to insulate translation when re-initiation is deleterious.
AB - In bacteria, translation re-initiation is crucial for synthesizing proteins encoded by genes that are organized into operons. The mechanisms regulating translation re-initiation remain, however, poorly understood. We now describe the ribosome termination structure (RTS), a conserved and stable mRNA secondary structure precisely localized downstream of stop codons, which serves as the main factor governing re-initiation efficiency in a synthetic Escherichia coli operon. We further report that in 95% of 128 analyzed bacterial genomes representing all phyla, this structure is selectively depleted when re-initiation is advantageous yet selectively enriched so as to insulate translation when re-initiation is deleterious.
UR - https://www.mendeley.com/catalogue/c4f8e661-68ba-3c95-b446-db4b968baf41/
U2 - 10.1101/2020.02.10.941153
DO - 10.1101/2020.02.10.941153
M3 - Preprint
BT - mRNA secondary structure stability regulates bacterial translation insulation and re-initiation
ER -