Multi-dimensional digital holographic microscopy

Osamu Matoba, Xiangyu Quan, Manoj Kumar, Yasuhiro Awatsuji

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations


In this paper, we present a multi-dimensional digital holographic microscopy for biological applications. First, we present our concept of an optical microscopy that can produce both phase and fluorescence images. Then, a technique is demonstrated to obtain the three-dimensional distribution of fluorescence beads by a common-path off-axis incoherent digital holographic microscopy. Experimental verification of the proposed system on microscopic samples and the reconstructed image quality is discussed.

Original languageEnglish
Title of host publicationOptical Design and Testing VIII
EditorsYongtian Wang, Tina E. Kidger, Kimio Tatsuno
ISBN (Electronic)9781510622289
StatePublished - 1 Jan 2018
Externally publishedYes
EventOptical Design and Testing VIII 2018 - Beijing, China
Duration: 11 Oct 201813 Oct 2018

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


ConferenceOptical Design and Testing VIII 2018


  • Multi-Dimensional digital holographic microscopy
  • incoherent digital holography
  • phase and fluorescence imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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