TY - JOUR
T1 - Multi-Site Incorporation of Nonstandard Amino Acids into Protein-Based Biomaterials
AU - Israeli, Bar
AU - Vaserman, Livne
AU - Amiram, Miriam
N1 - Publisher Copyright:
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2020/12/1
Y1 - 2020/12/1
N2 - The ability of natural biomaterials to shape, support, and orchestrate function inspires our efforts to produce functional materials. Guided by protein-based biomaterials, template-directed incorporation of synthetic building blocks, such as nonstandard amino acids (nsAAs), can expand the functions of biomaterials by endowing them with new physical and biophysical properties. In this short review, we describe existing technologies for multi-site nsAA incorporation into proteins. We then discuss examples of the application of this technology for engineering new functions in artificial biopolymers, for creating bio-inspired adhesives, and for improving the stability of biomaterials. We conclude by briefly discussing recent advances in synthetic biology that have the potential to expand our ability to design protein-based biomaterials composed of numerous and increasingly exotic nonstandard monomers.
AB - The ability of natural biomaterials to shape, support, and orchestrate function inspires our efforts to produce functional materials. Guided by protein-based biomaterials, template-directed incorporation of synthetic building blocks, such as nonstandard amino acids (nsAAs), can expand the functions of biomaterials by endowing them with new physical and biophysical properties. In this short review, we describe existing technologies for multi-site nsAA incorporation into proteins. We then discuss examples of the application of this technology for engineering new functions in artificial biopolymers, for creating bio-inspired adhesives, and for improving the stability of biomaterials. We conclude by briefly discussing recent advances in synthetic biology that have the potential to expand our ability to design protein-based biomaterials composed of numerous and increasingly exotic nonstandard monomers.
KW - biomaterials
KW - nonstandard amino acids
KW - orthogonal translation systems
KW - protein-polymers
KW - synthetic biology
UR - http://www.scopus.com/inward/record.url?scp=85073928414&partnerID=8YFLogxK
U2 - 10.1002/ijch.201900043
DO - 10.1002/ijch.201900043
M3 - Review article
AN - SCOPUS:85073928414
SN - 0021-2148
VL - 60
SP - 1118
EP - 1128
JO - Israel Journal of Chemistry
JF - Israel Journal of Chemistry
IS - 12
ER -