Multi-wavelength digital holographic microscopy for bio-imaging and applications

Manoj Kumar, Osamu Matoba, Xiangyu Quan, Yasuhiro Awatsuji

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


A single-shot common-path off-axis multi-wavelength digital holographic microscopic (MW-DHM) system for phase imaging of biological specimens is proposed. The DHM system is based on a cube beam splitter which divides the object beam into two beams: one beam is spatially filtered at its Fourier plane to make a reference spherical beam and another beam acts as the object beam. Three wavelengths: 473, 532, and 632.8 nm are used to illuminate the object under observation. The obtained experimental results on various objects corroborate the imaging capability of the proposed single-shot common-path off-axis multi-wavelength MW-DHM system. The proposed system may open new possibilities in bio-imaging and real-time simultaneous measurement of various parameters including the thickness, refractive index, etc.

Original languageEnglish
Title of host publicationBiomedical Imaging and Sensing Conference 2020
EditorsToyohiko Yatagai, Yoshihisa Aizu, Osamu Matoba, Yasuhiro Awatsuji, Yuan Luo
ISBN (Electronic)9781510638495
StatePublished - 1 Jan 2020
Externally publishedYes
Event6th Biomedical Imaging and Sensing Conference - Yokohama, Japan
Duration: 20 Apr 202024 Apr 2020

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


Conference6th Biomedical Imaging and Sensing Conference


  • Bio-imaging
  • Digital holographic microscopy
  • Phase imaging
  • Refractive index.

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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