Multimodal digital holographic microscopy

Manoj Kumar, Xiangyu Quan, Yasuhiro Awatsuji, Yosuke Tamada, Osamu Matob

Research output: Contribution to journalConference articlepeer-review


In this paper, we present a new multimodal approach for biological applications which comprises of a common-path off-axis incoherent digital holographic microscope to obtain 3D fluorescence imaging and another off-axis coherent digital holographic microscope to retrieve 3D phase imaging. The concept of the multimodal system to achieve both the 3D fluorescence and phase images is demonstrated on fluorescence beads and biological specimen. The experimentally obtained results authenticate the usefulness of the multimodal imaging system. The proposed system could found a beneficial role for numerous biomedical investigations.

Original languageEnglish
Article number1114001-59
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 1 Jan 2019
Externally publishedYes
EventBiomedical Imaging and Sensing Conference 2019, BISC 2019 - Yokohama, Japan
Duration: 22 Apr 201926 Apr 2019


  • 3D fluorescence imaging
  • Digital holographic microscopy
  • Multimodal imaging system
  • Phase imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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