Multimodal digital holographic microscopy

Manoj Kumar; Xiangyu Quan; Yasuhiro Awatsuji; Yosuke Tamada; Osamu Matob

Multimodal digital holographic microscopy

Manoj Kumar, Xiangyu Quan, Yasuhiro Awatsuji, Yosuke Tamada, Osamu Matob

Research output: Contribution to journal › Conference article › peer-review

Abstract

In this paper, we present a new multimodal approach for biological applications which comprises of a common-path off-axis incoherent digital holographic microscope to obtain 3D fluorescence imaging and another off-axis coherent digital holographic microscope to retrieve 3D phase imaging. The concept of the multimodal system to achieve both the 3D fluorescence and phase images is demonstrated on fluorescence beads and biological specimen. The experimentally obtained results authenticate the usefulness of the multimodal imaging system. The proposed system could found a beneficial role for numerous biomedical investigations.

Original language	English
Article number	1114001-59
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	11140
State	Published - 1 Jan 2019
Externally published	Yes
Event	Biomedical Imaging and Sensing Conference 2019, BISC 2019 - Yokohama, Japan Duration: 22 Apr 2019 → 26 Apr 2019

Keywords

3D fluorescence imaging
Digital holographic microscopy
Multimodal imaging system
Phase imaging

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Cite this

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title = "Multimodal digital holographic microscopy",

abstract = "In this paper, we present a new multimodal approach for biological applications which comprises of a common-path off-axis incoherent digital holographic microscope to obtain 3D fluorescence imaging and another off-axis coherent digital holographic microscope to retrieve 3D phase imaging. The concept of the multimodal system to achieve both the 3D fluorescence and phase images is demonstrated on fluorescence beads and biological specimen. The experimentally obtained results authenticate the usefulness of the multimodal imaging system. The proposed system could found a beneficial role for numerous biomedical investigations.",

keywords = "3D fluorescence imaging, Digital holographic microscopy, Multimodal imaging system, Phase imaging",

author = "Manoj Kumar and Xiangyu Quan and Yasuhiro Awatsuji and Yosuke Tamada and Osamu Matob",

note = "Publisher Copyright: {\textcopyright} 2019 SPIE.; Biomedical Imaging and Sensing Conference 2019, BISC 2019 ; Conference date: 22-04-2019 Through 26-04-2019",

year = "2019",

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TY - JOUR

T1 - Multimodal digital holographic microscopy

AU - Kumar, Manoj

AU - Quan, Xiangyu

AU - Awatsuji, Yasuhiro

AU - Tamada, Yosuke

AU - Matob, Osamu

PY - 2019/1/1

Y1 - 2019/1/1

N2 - In this paper, we present a new multimodal approach for biological applications which comprises of a common-path off-axis incoherent digital holographic microscope to obtain 3D fluorescence imaging and another off-axis coherent digital holographic microscope to retrieve 3D phase imaging. The concept of the multimodal system to achieve both the 3D fluorescence and phase images is demonstrated on fluorescence beads and biological specimen. The experimentally obtained results authenticate the usefulness of the multimodal imaging system. The proposed system could found a beneficial role for numerous biomedical investigations.

AB - In this paper, we present a new multimodal approach for biological applications which comprises of a common-path off-axis incoherent digital holographic microscope to obtain 3D fluorescence imaging and another off-axis coherent digital holographic microscope to retrieve 3D phase imaging. The concept of the multimodal system to achieve both the 3D fluorescence and phase images is demonstrated on fluorescence beads and biological specimen. The experimentally obtained results authenticate the usefulness of the multimodal imaging system. The proposed system could found a beneficial role for numerous biomedical investigations.

KW - 3D fluorescence imaging

KW - Digital holographic microscopy

KW - Multimodal imaging system

KW - Phase imaging

UR - http://www.scopus.com/inward/record.url?scp=85065730481&partnerID=8YFLogxK

M3 - Conference article

AN - SCOPUS:85065730481

SN - 0277-786X

VL - 11140

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

M1 - 1114001-59

T2 - Biomedical Imaging and Sensing Conference 2019, BISC 2019

Y2 - 22 April 2019 through 26 April 2019

ER -

Multimodal digital holographic microscopy

Abstract

Keywords

ASJC Scopus subject areas

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