Multimodal Microscopy: Fast Acquisition of Quantitative Phase and Fluorescence Imaging in 3D Space

Xiangyu Quan; Manoj Kumar; Sudheesh K. Rajput; Yosuke Tamada; Yasuhiro Awatsuji; Osamu Matoba

doi:10.1109/JSTQE.2020.3038403

Multimodal Microscopy: Fast Acquisition of Quantitative Phase and Fluorescence Imaging in 3D Space

Xiangyu Quan, Manoj Kumar, Sudheesh K. Rajput, Yosuke Tamada, Yasuhiro Awatsuji, Osamu Matoba

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Multimodal optical microscopy combines different optical and physiological principles working together to obtain rich perspectives from a single biological event. In this paper, the recent advances in multimodal microscopy systems based on off-axis digital holography and transport-of-intensity equation are reviewed. Both methods used the common-path configuration, and realized simultaneous quantitative phase and fluorescence imaging in 3D space. Feasibility checks with fluorescent micro-beads and live plant cells are presented. The results show that both methods are capable of fast acquisition of the quantitative phase and fluorescence information with digital refocusing ability.

Original language	English
Article number	9260959
Journal	IEEE Journal of Selected Topics in Quantum Electronics
Volume	27
Issue number	4
DOIs	https://doi.org/10.1109/JSTQE.2020.3038403
State	Published - 1 Jul 2021
Externally published	Yes

Keywords

Multimodal optical microscopy
digital holographic microscope
transport of intensity equation

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Electrical and Electronic Engineering

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10.1109/JSTQE.2020.3038403

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title = "Multimodal Microscopy: Fast Acquisition of Quantitative Phase and Fluorescence Imaging in 3D Space",

abstract = "Multimodal optical microscopy combines different optical and physiological principles working together to obtain rich perspectives from a single biological event. In this paper, the recent advances in multimodal microscopy systems based on off-axis digital holography and transport-of-intensity equation are reviewed. Both methods used the common-path configuration, and realized simultaneous quantitative phase and fluorescence imaging in 3D space. Feasibility checks with fluorescent micro-beads and live plant cells are presented. The results show that both methods are capable of fast acquisition of the quantitative phase and fluorescence information with digital refocusing ability.",

keywords = "Multimodal optical microscopy, digital holographic microscope, transport of intensity equation",

author = "Xiangyu Quan and Manoj Kumar and Rajput, \{Sudheesh K.\} and Yosuke Tamada and Yasuhiro Awatsuji and Osamu Matoba",

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T2 - Fast Acquisition of Quantitative Phase and Fluorescence Imaging in 3D Space

AU - Quan, Xiangyu

AU - Kumar, Manoj

AU - Rajput, Sudheesh K.

AU - Tamada, Yosuke

AU - Awatsuji, Yasuhiro

AU - Matoba, Osamu

PY - 2021/7/1

Y1 - 2021/7/1

N2 - Multimodal optical microscopy combines different optical and physiological principles working together to obtain rich perspectives from a single biological event. In this paper, the recent advances in multimodal microscopy systems based on off-axis digital holography and transport-of-intensity equation are reviewed. Both methods used the common-path configuration, and realized simultaneous quantitative phase and fluorescence imaging in 3D space. Feasibility checks with fluorescent micro-beads and live plant cells are presented. The results show that both methods are capable of fast acquisition of the quantitative phase and fluorescence information with digital refocusing ability.

AB - Multimodal optical microscopy combines different optical and physiological principles working together to obtain rich perspectives from a single biological event. In this paper, the recent advances in multimodal microscopy systems based on off-axis digital holography and transport-of-intensity equation are reviewed. Both methods used the common-path configuration, and realized simultaneous quantitative phase and fluorescence imaging in 3D space. Feasibility checks with fluorescent micro-beads and live plant cells are presented. The results show that both methods are capable of fast acquisition of the quantitative phase and fluorescence information with digital refocusing ability.

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KW - digital holographic microscope

KW - transport of intensity equation

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JO - IEEE Journal of Selected Topics in Quantum Electronics

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Multimodal Microscopy: Fast Acquisition of Quantitative Phase and Fluorescence Imaging in 3D Space

Abstract

Keywords

ASJC Scopus subject areas

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