Multiresistant Vibrio cholerae non-O1/non-O139 from waters in South India: Resistance patterns and virulence-associated gene profiles

Sankar Jagadeeshan, Praveen Kumar, Wilson Peter Abraham, Sabu Thomas

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

From different aquatic locations in Alleppey district, Kerala, South India a number (n = 36) of multiresistant non-O1, non-O139 V. cholerae strains were isolated. Water samples were filtered through 0.22 μm membrane filters, enriched in alkaline peptone water and plated onto thiosulfate-citrate-bile salts-sucrose (TCBS) agar. The isolates were resistance to cefotaxime (50%), nalidixic acid (44.4%), streptomycin and tetracycline (41.6%), trimethoprim (38.8%), cotrimoxazole (33.3%), furazolidone (27.7%), neomycin and ofloxacin (19.4%), ciprofloxacin, norfloxacin and spectinomycin (16.6%), gentamicin (8.3%) and chloramphenicol (2.7%). To our knowledge, this is the first report from Kerala, South India on the emergence of multiple drug resistance in V. cholerae isolates belonging to serogroup other than O1 and O139. Virulence-associated gene profiling of the isolates by PCR revealed the presence of toxR (100%), rtxA (61.1%), hlyA (50%), mshA (33.3%), tcpA-acfB (13.8%) and st (2.7%) genes. The virulence gene clusters ctxA, ompU, ace, and zot were not detected. This study demonstrates the presence of a wide array of critical virulence factors in diverse strains of Vibrio cholerae non-O1/non-O139. Hence, this serogroup can no longer be ignored as an environmental reservoir of virulence genes.

Original languageEnglish
Pages (from-to)538-544
Number of pages7
JournalJournal of Basic Microbiology
Volume49
Issue number6
DOIs
StatePublished - 1 Nov 2009
Externally publishedYes

Keywords

  • Cholera
  • Non-O139
  • Serogroup non-O1
  • Vibrio cholerae
  • Virulence-associated gene profiling

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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