Mutual effects of MinD-membrane interaction: II. Domain structure of the membrane enhances MinD binding

Shirley Mazor, Tomer Regev, Eugenia Mileykovskaya, William Margolin, William Dowhan, Itzhak Fishov

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


MinD, a well-conserved bacterial amphitropic protein involved in spatial regulation of cell division, has a typical feature of reversible binding to the membrane. MinD shows a clear preference for acidic phospholipids organized into lipid domains in bacterial membrane. We have shown that binding of MinD may change the dynamics of model and native membranes (see accompanying paper [1]). On the other hand, MinD dimerization and anchoring could be enhanced on pre-existing anionic phospholipid domains. We have tested MinD binding to model membranes in which acidic and zwitterionic phospholipids are either well-mixed or segregated to phase domains. The phase separation was achieved in binary mixtures of 1-Stearoyl-2-Oleoyl-sn-Glycero-3-[Phospho-rac-(1-glycerol] (SOPG) with 1,2-Distearoyl-sn-Glycero-3-Phosphocholine (DSPC) or 1,2-Distearoyl-sn-Glycero-3-[Phospho-rac-(1-glycerol)] (DSPG) and binding to these membranes was compared with that to a fluid mixture of SOPG with 1-Stearoyl-2-Oleoyl-sn-Glycero-3-Phosphocholine (SOPC). The results demonstrate that MinD binding to the membrane is enhanced by segregation of anionic phospholipids to fluid domains in a gel-phase environment and, moreover, the protein stabilizes such domains. This suggests that an uneven binding of MinD to the heterogeneous native membrane is possible, leading to formation of a lipid-specific distribution pattern of MinD and/or modulation of its temporal behavior.

Original languageEnglish
Pages (from-to)2505-2511
Number of pages7
JournalBiochimica et Biophysica Acta - Biomembranes
Issue number11
StatePublished - 1 Nov 2008


  • Membrane domain
  • Phase transition
  • Protein-membrane interaction
  • α helix

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology


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