Mycophenolate mofetil alone and in combination with tacrolimus inhibits the proliferation of HT-29 human colonic adenocarcinoma cell line and might interfere with colonic tumorigenesis

Galina Ling, Sergio Lamprecht, George Shubinsky, Lidia Osyntsov, Baruch Yerushalmi, Ilya Pinsk, Vered Pinsk, Eduard Ling

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Background/Aim: Familial adenomatous polyposis (FAP) was found to be completely reversed in a patient treated with mycophenolate mofetil (MMF) and tacrolimus following kidney transplantation. In this preliminary study, we assessed whether MMF and tacrolimus alone or in combination interfere with the cell cycle and proliferation in a human colonic adenocarcinoma cell line and in the colonic polyps of the patient with FAP. Materials and Methods: Human colonic adenocarcinoma HT-29 cells were treated with tacrolimus and MMF alone and in combination at different concentrations. Cell viability and proliferation were assessed using the MTT assay. Cell-cycle distribution was analyzed by flow cytometry. Expression of Ki-67, a marker of mitotic activity, was evaluated in the patient’s colonic polyps before and under drug treatment. Results: MMF in combination with tacrolimus induced S-phase cell-cycle arrest and markedly inhibited HT-29 cell proliferation. Ki-67 expression in the patient’s colonic polyps was significantly reduced following combined tacrolimus and MMF treatment. Conclusion: MMF and tacrolimus synergistically inhibited proliferation of a human colonic adenocarcinoma cell line and interfered with the expansion of colonic crypt proliferation in the polyp from a patient with FAP. The results confirm our clinical observation and indicate the possibility of novel approach to therapy of colorectal neoplasia.

Original languageEnglish
Pages (from-to)3333-3339
Number of pages7
JournalAnticancer Research
Volume38
Issue number6
DOIs
StatePublished - 1 Jun 2018

Keywords

  • Antiproliferative effect
  • HT-29 cells
  • MMF
  • Synergism
  • Tacrolimus

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint

Dive into the research topics of 'Mycophenolate mofetil alone and in combination with tacrolimus inhibits the proliferation of HT-29 human colonic adenocarcinoma cell line and might interfere with colonic tumorigenesis'. Together they form a unique fingerprint.

Cite this