Mycorrhized Ri-transformed roots facilitate in vitro inoculation of Cistus incanus with Tuber melanosporum

Yvonne Ventura, David Mills, Varda Kagan-Zur, Nurit Roth-Bejerano, Amnon Bustan

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Progress was made towards a reliable in vitro system for mycorrhizing Cistus incanus seedlings with Tuber melanosporum. A rich growth medium favored extensive growth of mycorrhized Ri-transformed roots (MTR) but inhibited mycelial outgrowth into the medium. A minimal medium, on the other hand, inhibited MTR growth but supported considerable mycelial outgrowth into the medium. While the presence of a C. incanus propagule clearly enhanced mycelial growth into the minimal medium, a highly significant factor appeared to be the use of MTR inoculant, which supported mycorrhizal development to the Hartig net stage. The advantages of MTR for in vitro mycorrhization of host plant seedlings are discussed.

Original languageEnglish
Pages (from-to)53-61
Number of pages9
JournalPlant Cell, Tissue and Organ Culture
Volume85
Issue number1
DOIs
StatePublished - 1 Apr 2006

Keywords

  • Black Périgord truffle
  • Hairy roots
  • Inoculation method
  • Mycorrhiza

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