Mycorrhized Ri-transformed roots facilitate in vitro inoculation of Cistus incanus with Tuber melanosporum

Yvonne Ventura; David Mills; Varda Kagan-Zur; Nurit Roth-Bejerano; Amnon Bustan

doi:10.1007/s11240-005-9048-0

Mycorrhized Ri-transformed roots facilitate in vitro inoculation of Cistus incanus with Tuber melanosporum

Yvonne Ventura, David Mills, Varda Kagan-Zur, Nurit Roth-Bejerano, Amnon Bustan

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Progress was made towards a reliable in vitro system for mycorrhizing Cistus incanus seedlings with Tuber melanosporum. A rich growth medium favored extensive growth of mycorrhized Ri-transformed roots (MTR) but inhibited mycelial outgrowth into the medium. A minimal medium, on the other hand, inhibited MTR growth but supported considerable mycelial outgrowth into the medium. While the presence of a C. incanus propagule clearly enhanced mycelial growth into the minimal medium, a highly significant factor appeared to be the use of MTR inoculant, which supported mycorrhizal development to the Hartig net stage. The advantages of MTR for in vitro mycorrhization of host plant seedlings are discussed.

Original language	English
Pages (from-to)	53-61
Number of pages	9
Journal	Plant Cell, Tissue and Organ Culture
Volume	85
Issue number	1
DOIs	https://doi.org/10.1007/s11240-005-9048-0
State	Published - 1 Apr 2006

Keywords

Black Périgord truffle
Hairy roots
Inoculation method
Mycorrhiza

ASJC Scopus subject areas

Horticulture

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10.1007/s11240-005-9048-0

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title = "Mycorrhized Ri-transformed roots facilitate in vitro inoculation of Cistus incanus with Tuber melanosporum",

abstract = "Progress was made towards a reliable in vitro system for mycorrhizing Cistus incanus seedlings with Tuber melanosporum. A rich growth medium favored extensive growth of mycorrhized Ri-transformed roots (MTR) but inhibited mycelial outgrowth into the medium. A minimal medium, on the other hand, inhibited MTR growth but supported considerable mycelial outgrowth into the medium. While the presence of a C. incanus propagule clearly enhanced mycelial growth into the minimal medium, a highly significant factor appeared to be the use of MTR inoculant, which supported mycorrhizal development to the Hartig net stage. The advantages of MTR for in vitro mycorrhization of host plant seedlings are discussed.",

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T1 - Mycorrhized Ri-transformed roots facilitate in vitro inoculation of Cistus incanus with Tuber melanosporum

AU - Ventura, Yvonne

AU - Mills, David

AU - Kagan-Zur, Varda

AU - Roth-Bejerano, Nurit

AU - Bustan, Amnon

PY - 2006/4/1

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N2 - Progress was made towards a reliable in vitro system for mycorrhizing Cistus incanus seedlings with Tuber melanosporum. A rich growth medium favored extensive growth of mycorrhized Ri-transformed roots (MTR) but inhibited mycelial outgrowth into the medium. A minimal medium, on the other hand, inhibited MTR growth but supported considerable mycelial outgrowth into the medium. While the presence of a C. incanus propagule clearly enhanced mycelial growth into the minimal medium, a highly significant factor appeared to be the use of MTR inoculant, which supported mycorrhizal development to the Hartig net stage. The advantages of MTR for in vitro mycorrhization of host plant seedlings are discussed.

AB - Progress was made towards a reliable in vitro system for mycorrhizing Cistus incanus seedlings with Tuber melanosporum. A rich growth medium favored extensive growth of mycorrhized Ri-transformed roots (MTR) but inhibited mycelial outgrowth into the medium. A minimal medium, on the other hand, inhibited MTR growth but supported considerable mycelial outgrowth into the medium. While the presence of a C. incanus propagule clearly enhanced mycelial growth into the minimal medium, a highly significant factor appeared to be the use of MTR inoculant, which supported mycorrhizal development to the Hartig net stage. The advantages of MTR for in vitro mycorrhization of host plant seedlings are discussed.

KW - Black Périgord truffle

KW - Hairy roots

KW - Inoculation method

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Mycorrhized Ri-transformed roots facilitate in vitro inoculation of Cistus incanus with Tuber melanosporum

Abstract

Keywords

ASJC Scopus subject areas

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