N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier

Ziqiang Guan, Antonia Delago, Phillip Nußbaum, Benjamin Meyer, Sonja Verena Albers, Jerry Eichler

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

N-glycosylation is a post-translational modification that occurs across evolution. In the thermoacidophilic archaea Sulfolobus acidocaldarius, glycoproteins are modified by an N-linked tribranched hexasaccharide reminiscent of the N-glycans assembled in Eukarya. Previously, hexose-bearing dolichol phosphate was detected in a S. acidocaldarius Bligh–Dyer lipid extract. Here, we used a specialized protocol for extracting lipid-linked oligosaccharides to detect a dolichol pyrophosphate bearing the intact hexasaccharide, as well as its biosynthetic intermediates. Furthermore, evidence for N-glycosylation of two S. acidocaldarius proteins by the same hexasaccharide and its derivatives was collected. These findings thus provide novel insight into archaeal N-glycosylation.

Original languageEnglish
Pages (from-to)3168-3178
Number of pages11
JournalFEBS Letters
Volume590
Issue number18
DOIs
StatePublished - 1 Sep 2016

Keywords

  • N-glycosylation
  • Sulfolobus acidocaldarius
  • archaea
  • dolichol
  • thermoacidophile

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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