N-methylation of isopeptide bond as a strategy to resist deubiquitinases

Mahmood Haj-Yahya, Najat Eltarteer, Shimrit Ohayon, Efrat Shema, Eran Kotler, Moshe Oren, Ashraf Brik

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Stable like a rock: An efficient method to generate N-methylated isopeptide bonds has been developed. The strategy was used to generate highly stable ubiquitinated peptides and proteins that are resistant to deubiquitinases (DUBs; see scheme). Thus, the behavior of several stable ubiquitin conjugates with different DUBs was studied in-vitro and within a cellular environment.

Original languageEnglish
Pages (from-to)11535-11539
Number of pages5
JournalAngewandte Chemie - International Edition
Issue number46
StatePublished - 12 Nov 2012


  • N-methylation
  • deubiquitination
  • histone synthesis
  • stable isopeptide bond
  • ubiquitination

ASJC Scopus subject areas

  • Catalysis
  • Chemistry (all)


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