Nano-suspension of ursolic acid for improving oral bioavailability and attenuation of type II diabetes: A histopathological investigation

Anil Kumar Singh, Himanshu Pandey, Pramod W. Ramteke, Shanti Bhushan Mishra

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Ursolic acid is a pentacyclic carboxylic acid present in medicinal herbs abundantly. There is scientific evidence of important benefits of ursolic acid in metabolism of lipids and glucose, as well as on the body weight and metabolic parameters. However, the hydrophobicity of ursolic acid increases the difficulty in its potential clinical application. The aim of the present study is to develop ursolic acid nanoparticles to enhance its bioavailability and further subjected for evaluating the antidiabetic activity. Ursolic acid nanoparticles were prepared by nanoprecipitation method and optimized by varying formulation variables including PVA concentration and processing variables including stirring speed. Mean Particle Size, Polydispersity index and zeta potential were measured for the optimized nanoparticles. Non-insulin dependent diabetes mellitus was induced intra-peritonially in wistar albino rats at 60 mg/kg streptozotocin. Ursolic acid (100 mg/kg) and nanoparticle (UNP 25 mg/kg and 50 mg/kg) were given orally for 4 weeks. In vivo antidiabetic effect was estimated by measuring blood glucose level. The Serum cholesterol, triglycerides, SGOT, SGPT, albumin, total protein and in-vivo antioxidant parameters were determined by using diagnostic kits. Optimized Ursolic acid nanosuspension showed particle size and polydispersity index to be 246.4 nm and 0.206 respectively. Zeta potential of the prepared nanoparticle was −31.2 ± 5.17 mV. Ursolic acid nanoparticle showed significant reduction (p < 0.001) in elevated blood glucose level in dose dependent manner with prominent lipid lowering and antioxidant effect. The promising results from the study revealed that nano-preparation was found to be the most effective for antidiabetic activity due to its changes in oxidative biomarkers.

Original languageEnglish
Article number101433
JournalBiocatalysis and Agricultural Biotechnology
Volume22
DOIs
StatePublished - 1 Nov 2019
Externally publishedYes

Keywords

  • Antidiabetic
  • Antioxidant
  • Nanoparticle
  • Streptozotocin
  • Ursolic acid

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science

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