Nanolithography Using Protease Etching of Protein Surfaces

Rodica E. Ionescu, Robert S. Marks, Levi A. Gheber

    Research output: Contribution to journalArticlepeer-review

    30 Scopus citations


    We present for the first time the use of a proteolytic enzyme to create nanometer-size depressions in a protein surface. This was achieved by delivering trypsin via a nanopipet, controlled with a scanning probe microscope, to a dried bovine serum albumin film. We suggest a comprehensive model, explaining the processes involved and providing ways to control the parameters governing them. Enzyme nanolithography should be useful in areas of nanobiotechnology, such as nanobiochips, nanofluidics, and lab-on-a-chip.

    Original languageEnglish
    Pages (from-to)1639-1642
    Number of pages4
    JournalNano Letters
    Issue number12
    StatePublished - 1 Dec 2003

    ASJC Scopus subject areas

    • Bioengineering
    • General Chemistry
    • General Materials Science
    • Condensed Matter Physics
    • Mechanical Engineering


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