@article{903254c9e62b47f3a422e46bb2edc83a,
title = "Nanoscale resolution of microbial fiber degradation in action",
abstract = "The lives of microbes unfold at the micron scale, and their molecular machineries operate at the nanoscale. Their study at these resolutions is key towards achieving a better understanding of their ecology. We focus on cellulose degradation of the canonical Clostridium thermocellum system to comprehend how microbes build and use their cellulosomal machinery at these nanometer scales. Degradation of cellulose, the most abundant organic polymer on Earth, is instrumental to the global carbon cycle. We reveal that bacterial cells form {"}cellulosome capsules{"} driven by catalytic product-dependent dynamics, which can increase the rate of hydrolysis. Biosynthesis of this energetically costly machinery and cell growth are decoupled at the single-cell level, hinting at a division-of-labor strategy through phenotypic heterogeneity. This novel observation highlights intra-population interactions as key to understanding rates of fiber degradation.",
keywords = "cellulosome, cohesin, cryo-EM and cryo-ET, division of labor, dockerin, phenotypic heterogeneity, scaffoldin",
author = "Meltem Tatlı and Sarah Mora{\"i}s and Tovar-Herrera, {Omar E.} and Bomble, {Yannick J.} and Bayer, {Edward A.} and Ohad Medalia and Itzhak Mizrahi",
note = "Funding Information: This work was funded by the DIP (2476/2-1) to I.M and O.M., ERC (64084, to I.M), Swiss national foundation grant (SNSF 31003A_179418) to O.M. SM is grateful to EMBO for the short-term fellowship (7686) allowing a visit to the University of Zurich. Funding was also provided by the Center for Bioenergy Innovation (CBI), a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. The authors thank Eva Setter-Lamed (Weizmann Institute of Sciences, Israel) for technical assistance, Matthias Wojtynek (UZH/ETH) for assisting with template matching procedures, Rebecca de Leeuw (UZH) and Miriam Weber (UZH). The authors thank the center of microscopy and image analysis (ZMB) at the university of Zurich, and the Ilse Katz Institute for Nanoscale Science and Technology Shared Resource Facility under the direction of Dr. Uzi Hadad for image acquisition with a Zeiss LSM880 Airyscan. The authors are also grateful to Prof. Otto X. Cordero (MIT) for providing constructive criticisms during the final stages of manuscript preparation. Funding Information: This work was funded by the DIP (2476/2 -1) to I.M and O.M., ERC (64084, to I.M), Swiss national foundation grant (SNSF 31003A_179418) to O.M. SM is grateful to EMBO for the short-term fellowship (7686) allowing a visit to the University of Zurich. Funding was also provided by the Center for Bioenergy Innovation (CBI), a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. The authors thank Eva Setter-Lamed (Weizmann Institute of Sciences, Israel) for technical assistance, Matthias Wojtynek (UZH/ETH) for assisting with template matching procedures, Rebecca de Leeuw (UZH) and Miriam Weber (UZH). The authors thank the center of microscopy and image analysis (ZMB) at the university of Zurich, and the Ilse Katz Institute for Nanoscale Science and Technology Shared Resource Facility under the direction of Dr. Uzi Hadad for image acquisition with a Zeiss LSM880 Airyscan. The authors are also grateful to Prof. Otto X. Cordero (MIT) for providing constructive criticisms during the final stages of manuscript preparation. Publisher Copyright: {\textcopyright} 2022, eLife Sciences Publications Ltd. All rights reserved.",
year = "2022",
month = may,
day = "1",
doi = "10.7554/eLife.76523",
language = "English",
volume = "11",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}