TY - GEN
T1 - Nanoscuptured Thin Films Based SERS Nanobiosensor for Highly Sensitive and Specific Detection of E. coli
AU - Srivastava, Sachin K.
AU - Ben-Hamo, Hilla
AU - Kushmaro, Ariel
AU - Marks, Robert S.
AU - Grüner, Christoph
AU - Rauschenbach, Bernd
AU - Abdulhalim, Ibrahim
PY - 2015/6/21
Y1 - 2015/6/21
N2 - We have fabricated a surface enhanced Raman spectroscopy (SERS) based nanobiosensor for very specific detection of E. coli up to the concentration levels of single bacterium. The sensor was fabricated on nanosculptured thin films (nSTFs) of Ag over Si substrate. The height, porosity and other features of the nSTF were optimized for the maximum enhancement in our previous studies [1, 2]. T4- bacteriophages were immobilized on the surface of the nSTF by using 4-aminothiophenol (4-ATP) and glutaraldehyde as a cross-linker. Further, the sensor chip was incubated in bovine serum albumin solution to block the sites available for nonspecific binding. The sample solutions of varying concentrations of two strains of E. coli (RFM443 (E. coli B) and XLMRF (E. coli μX)] and 3 other gram negative bacteria C. violaceum (CV026), P. dentrificans and P. aeruginosa were prepared for the characterization of the sensor probe. The bacteria other than E. coli B were used for control experiments to demonstrate the specificity of the sensor.
AB - We have fabricated a surface enhanced Raman spectroscopy (SERS) based nanobiosensor for very specific detection of E. coli up to the concentration levels of single bacterium. The sensor was fabricated on nanosculptured thin films (nSTFs) of Ag over Si substrate. The height, porosity and other features of the nSTF were optimized for the maximum enhancement in our previous studies [1, 2]. T4- bacteriophages were immobilized on the surface of the nSTF by using 4-aminothiophenol (4-ATP) and glutaraldehyde as a cross-linker. Further, the sensor chip was incubated in bovine serum albumin solution to block the sites available for nonspecific binding. The sample solutions of varying concentrations of two strains of E. coli (RFM443 (E. coli B) and XLMRF (E. coli μX)] and 3 other gram negative bacteria C. violaceum (CV026), P. dentrificans and P. aeruginosa were prepared for the characterization of the sensor probe. The bacteria other than E. coli B were used for control experiments to demonstrate the specificity of the sensor.
UR - http://www.scopus.com/inward/record.url?scp=85019530318&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85165748649
SN - 9781467374750
T3 - Optics InfoBase Conference Papers
BT - European Conference on Lasers and Electro-Optics, CLEO 2015
PB - Optical Society of America (OSA)
T2 - 2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference, CLEO/Europe-EQEC 2015
Y2 - 21 June 2015 through 25 June 2015
ER -