Nanoscuptured Thin Films Based SERS Nanobiosensor for Highly Sensitive and Specific Detection of E. coli

Sachin K. Srivastava, Hilla Ben Hamo, Ariel Kushmaro, Robert S. Marks, Christoph Grüner, Bernd Rauschenbach, Ibrahim Abdulhalim

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We have fabricated a surface enhanced Raman spectroscopy (SERS) based nanobiosensor for very specific detection of E. coli up to the concentration levels of single bacterium. The sensor was fabricated on nanosculptured thin films (nSTFs) of Ag over Si substrate. The height, porosity and other features of the nSTF were optimized for the maximum enhancement in our previous studies [1, 2]. T4- bacteriophages were immobilized on the surface of the nSTF by using 4-aminothiophenol (4-ATP) and glutaraldehyde as a cross-linker. Further, the sensor chip was incubated in bovine serum albumin solution to block the sites available for nonspecific binding. The sample solutions of varying concentrations of two strains of E. coli (RFM443 (E. coli B) and XLMRF (E. coli μX)] and 3 other gram negative bacteria C. violaceum (CV026), P. dentrificans and P. aeruginosa were prepared for the characterization of the sensor probe. The bacteria other than E. coli B were used for control experiments to demonstrate the specificity of the sensor.
Original languageEnglish
Title of host publicationEuropean Conference on Lasers and Electro-Optics, CLEO 2015
PublisherOptical Society of America (OSA)
StatePublished - 21 Jun 2015
Event2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference, CLEO/Europe-EQEC 2015 - Munich, Germany
Duration: 21 Jun 201525 Jun 2015

Publication series

NameOptics InfoBase Conference Papers

Conference

Conference2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference, CLEO/Europe-EQEC 2015
Country/TerritoryGermany
CityMunich
Period21/06/1525/06/15

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Mechanics of Materials

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