Nanosheets Based Approach to Elevate the Proliferative and Differentiation Efficacy of Human Wharton’s Jelly Mesenchymal Stem Cells

Suraj Kumar Singh, Anshuman Singh, Vinod Kumar, Jalaj Gupta, Sima Umrao, Manoj Kumar, Devojit Kumar Sarma, Marcis Leja, Manohar Prasad Bhandari, Vinod Verma

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Mesenchymal stem cell (MSC)-based therapy and tissue repair necessitate the use of an ideal clinical biomaterial capable of increasing cell proliferation and differentiation. Recently, MXenes 2D nanomaterials have shown remarkable potential for improving the functional properties of MSCs. In the present study, we elucidated the potential of Ti2CTx MXene as a biomaterial through its primary biological response to human Wharton’s Jelly MSCs (hWJ-MSCs). A Ti2CTx nanosheet was synthesized and thoroughly characterized using various microscopic and spectro-scopic tools. Our findings suggest that Ti2CTx MXene nanosheet exposure does not alter the morphology of the hWJ-MSCs; however, it causes a dose-dependent (10–200 µg/mL) increase in cell proliferation, and upon using it with conditional media, it also enhanced its tri-lineage differentiation potential, which is a novel finding of our study. A two-fold increase in cell viability was also noticed at the highest tested dose of the nanosheet. The treated hWJ-MSCs showed no sign of cellular stress or toxicity. Taken together, these findings suggest that the Ti2CTx MXene nanosheet is capable of augmenting the proliferation and differentiation potential of the cells.

Original languageEnglish
Article number5816
JournalInternational Journal of Molecular Sciences
Volume23
Issue number10
DOIs
StatePublished - 1 May 2022
Externally publishedYes

Keywords

  • Ti2CTx MXene
  • cell differentiation
  • cell proliferation
  • cell viability
  • hWJ-MSCs
  • nanosheets
  • stemness

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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