Native chemical ligation at glutamine

Peter Siman, Subramanian Vedhanarayanan Karthikeyan, Ashraf Brik

Research output: Contribution to journalArticlepeer-review

78 Scopus citations


The desulfurization reaction introduced by Yan and Dawson as a postnative chemical ligation step greatly expanded the scope of ligation chemistry beyond Xaa-Cys (Xaa is any amino acid) by making ligation at Xaa-Phe, Xaa-Val, Xaa-Lys, Xaa-Leu, Xaa-Thr, and Xaa-Pro junctions accessible in the synthesis of functional proteins. A new ligation site based on Xaa-Gln utilizing γ-mercaptoglutamine is reported, and several examples on the efficiency of ligation coupled with desulfurization are provided.

Original languageEnglish
Pages (from-to)1520-1523
Number of pages4
JournalOrganic Letters
Issue number6
StatePublished - 16 Mar 2012

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry


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