Abstract
A natural IgM monoclonal antibody, 1.67, was generated from apparently healthy unstimulated BALB/c mice. This antibody reacted with L5178Y murine T cell lymphoma, with human Raji cells, and with several normal cells. Further analysis of its ligand binding capacity disclosed strong binding to single-stranded DNA (ssDNA). However, this naturally-occurring monoclonal antibody binds to different epitopes on cell membranes and on DNA than another anti-DNA monoclonal antibody (18/103/1) from human origin. This conclusion was based on competition assays. Furthermore, NOA 1.67 lacks the 16/6 idiotype expressed on the 18/103/1 antibody. The 16/6 idiotype is shared by human and mouse lupus monoclonal autoantibodies that bind simultaneously to lymphoid cells and DNA. This is a first report on a natural autoantibody that binds to malignant and to normal cell membrane(s) as well as to ssDNA. It may have regulatory functions controlling malignancy and or autoimmunity.
Original language | English |
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Pages (from-to) | 43-49 |
Number of pages | 7 |
Journal | Immunology Letters |
Volume | 18 |
Issue number | 1 |
DOIs | |
State | Published - 1 Jan 1988 |
Keywords
- Anti-DNA antibodies
- Autoantibodies
- Autoimmunity
- Natural antibodies
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology