Near-isogenic lines for measuring phenotypic effects of DIMBOA-Glc methyltransferase activity in maize

Valeria Mijares; Lisa N. Meihls; Georg Jander; Vered Tzin

doi:10.4161/psb.26779

Near-isogenic lines for measuring phenotypic effects of DIMBOA-Glc methyltransferase activity in maize

Valeria Mijares, Lisa N. Meihls, Georg Jander, Vered Tzin

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Three O-methyltransferases (BX10a, b, c) catalyze the conversion of 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one glucoside (DIMBOA-Glc) to 2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside (HDMBOA-Glc) in maize (Zea mays). Variation in benzoxazinoid accumulation and resistance to Rhopalosiphum maidis (corn leaf aphid) was attributed to a natural CACTA family transposon insertion that inactivates Bx10c. Whereas maize inbred line B73 has this transposon insertion, line CML277 does not. To characterize the phenotypic effects of DIMBOA-Glc methyltransferase activity, we created near-isogenic lines derived from B73 and CML277 that do or do not contain the transposon insertion. B×10c inactivation causes high DIMBOA-Glc, low HDMBOA-Glc, and decreased aphid reproduction relative to near-isogenic lines that have a functional B×10c gene. These results confirm the importance of this locus in maize aphid resistance. The availability of B×10c near-isogenic lines will facilitate further research on the function of different benzoxazinoids and DIMBOA-Glc methyltransferase activity in maize defense against herbivores and pathogens.

Original language	English
Article number	e26779
Journal	Plant Signaling and Behavior
Volume	8
Issue number	10
DOIs	https://doi.org/10.4161/psb.26779
State	Published - 25 Nov 2013
Externally published	Yes

Keywords

Benzoxazinoid
DIMBOA
HDMBOA
Rhopalosiphum maidis
Zea mays

ASJC Scopus subject areas

Plant Science

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10.4161/psb.26779

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@article{47835e4b6d164704bf8c1b4baf0106d7,

title = "Near-isogenic lines for measuring phenotypic effects of DIMBOA-Glc methyltransferase activity in maize",

abstract = "Three O-methyltransferases (BX10a, b, c) catalyze the conversion of 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one glucoside (DIMBOA-Glc) to 2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside (HDMBOA-Glc) in maize (Zea mays). Variation in benzoxazinoid accumulation and resistance to Rhopalosiphum maidis (corn leaf aphid) was attributed to a natural CACTA family transposon insertion that inactivates Bx10c. Whereas maize inbred line B73 has this transposon insertion, line CML277 does not. To characterize the phenotypic effects of DIMBOA-Glc methyltransferase activity, we created near-isogenic lines derived from B73 and CML277 that do or do not contain the transposon insertion. B×10c inactivation causes high DIMBOA-Glc, low HDMBOA-Glc, and decreased aphid reproduction relative to near-isogenic lines that have a functional B×10c gene. These results confirm the importance of this locus in maize aphid resistance. The availability of B×10c near-isogenic lines will facilitate further research on the function of different benzoxazinoids and DIMBOA-Glc methyltransferase activity in maize defense against herbivores and pathogens.",

keywords = "Benzoxazinoid, DIMBOA, HDMBOA, Rhopalosiphum maidis, Zea mays",

author = "Valeria Mijares and Meihls, {Lisa N.} and Georg Jander and Vered Tzin",

note = "Funding Information: This research was funded by US National Science Foundation Research Experience for Undergraduates grant DBI-1061199 for Mijares V, US National Science Foundation award IOS-1139329 to Jander G, United States Department of Agriculture award 2011-67012-30675 to Meihls LN, and Vaadia-BARD Postdoctoral Fellowship Award FI-471-2012 to Tzin V.",

year = "2013",

month = nov,

day = "25",

doi = "10.4161/psb.26779",

language = "English",

volume = "8",

journal = "Plant Signaling and Behavior",

issn = "1559-2316",

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TY - JOUR

T1 - Near-isogenic lines for measuring phenotypic effects of DIMBOA-Glc methyltransferase activity in maize

AU - Mijares, Valeria

AU - Meihls, Lisa N.

AU - Jander, Georg

AU - Tzin, Vered

N1 - Funding Information: This research was funded by US National Science Foundation Research Experience for Undergraduates grant DBI-1061199 for Mijares V, US National Science Foundation award IOS-1139329 to Jander G, United States Department of Agriculture award 2011-67012-30675 to Meihls LN, and Vaadia-BARD Postdoctoral Fellowship Award FI-471-2012 to Tzin V.

PY - 2013/11/25

Y1 - 2013/11/25

N2 - Three O-methyltransferases (BX10a, b, c) catalyze the conversion of 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one glucoside (DIMBOA-Glc) to 2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside (HDMBOA-Glc) in maize (Zea mays). Variation in benzoxazinoid accumulation and resistance to Rhopalosiphum maidis (corn leaf aphid) was attributed to a natural CACTA family transposon insertion that inactivates Bx10c. Whereas maize inbred line B73 has this transposon insertion, line CML277 does not. To characterize the phenotypic effects of DIMBOA-Glc methyltransferase activity, we created near-isogenic lines derived from B73 and CML277 that do or do not contain the transposon insertion. B×10c inactivation causes high DIMBOA-Glc, low HDMBOA-Glc, and decreased aphid reproduction relative to near-isogenic lines that have a functional B×10c gene. These results confirm the importance of this locus in maize aphid resistance. The availability of B×10c near-isogenic lines will facilitate further research on the function of different benzoxazinoids and DIMBOA-Glc methyltransferase activity in maize defense against herbivores and pathogens.

AB - Three O-methyltransferases (BX10a, b, c) catalyze the conversion of 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one glucoside (DIMBOA-Glc) to 2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside (HDMBOA-Glc) in maize (Zea mays). Variation in benzoxazinoid accumulation and resistance to Rhopalosiphum maidis (corn leaf aphid) was attributed to a natural CACTA family transposon insertion that inactivates Bx10c. Whereas maize inbred line B73 has this transposon insertion, line CML277 does not. To characterize the phenotypic effects of DIMBOA-Glc methyltransferase activity, we created near-isogenic lines derived from B73 and CML277 that do or do not contain the transposon insertion. B×10c inactivation causes high DIMBOA-Glc, low HDMBOA-Glc, and decreased aphid reproduction relative to near-isogenic lines that have a functional B×10c gene. These results confirm the importance of this locus in maize aphid resistance. The availability of B×10c near-isogenic lines will facilitate further research on the function of different benzoxazinoids and DIMBOA-Glc methyltransferase activity in maize defense against herbivores and pathogens.

KW - Benzoxazinoid

KW - DIMBOA

KW - HDMBOA

KW - Rhopalosiphum maidis

KW - Zea mays

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U2 - 10.4161/psb.26779

DO - 10.4161/psb.26779

M3 - Article

C2 - 24494232

AN - SCOPUS:84887895082

SN - 1559-2316

VL - 8

JO - Plant Signaling and Behavior

JF - Plant Signaling and Behavior

IS - 10

M1 - e26779

ER -

Near-isogenic lines for measuring phenotypic effects of DIMBOA-Glc methyltransferase activity in maize

Abstract

Keywords

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