Abstract
Three O-methyltransferases (BX10a, b, c) catalyze the conversion of 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one glucoside (DIMBOA-Glc) to 2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside (HDMBOA-Glc) in maize (Zea mays). Variation in benzoxazinoid accumulation and resistance to Rhopalosiphum maidis (corn leaf aphid) was attributed to a natural CACTA family transposon insertion that inactivates Bx10c. Whereas maize inbred line B73 has this transposon insertion, line CML277 does not. To characterize the phenotypic effects of DIMBOA-Glc methyltransferase activity, we created near-isogenic lines derived from B73 and CML277 that do or do not contain the transposon insertion. B×10c inactivation causes high DIMBOA-Glc, low HDMBOA-Glc, and decreased aphid reproduction relative to near-isogenic lines that have a functional B×10c gene. These results confirm the importance of this locus in maize aphid resistance. The availability of B×10c near-isogenic lines will facilitate further research on the function of different benzoxazinoids and DIMBOA-Glc methyltransferase activity in maize defense against herbivores and pathogens.
Original language | English |
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Article number | e26779 |
Journal | Plant Signaling and Behavior |
Volume | 8 |
Issue number | 10 |
DOIs | |
State | Published - 25 Nov 2013 |
Externally published | Yes |
Keywords
- Benzoxazinoid
- DIMBOA
- HDMBOA
- Rhopalosiphum maidis
- Zea mays
ASJC Scopus subject areas
- Plant Science