Near-isogenic lines for measuring phenotypic effects of DIMBOA-Glc methyltransferase activity in maize

Valeria Mijares, Lisa N. Meihls, Georg Jander, Vered Tzin

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Three O-methyltransferases (BX10a, b, c) catalyze the conversion of 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one glucoside (DIMBOA-Glc) to 2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside (HDMBOA-Glc) in maize (Zea mays). Variation in benzoxazinoid accumulation and resistance to Rhopalosiphum maidis (corn leaf aphid) was attributed to a natural CACTA family transposon insertion that inactivates Bx10c. Whereas maize inbred line B73 has this transposon insertion, line CML277 does not. To characterize the phenotypic effects of DIMBOA-Glc methyltransferase activity, we created near-isogenic lines derived from B73 and CML277 that do or do not contain the transposon insertion. B×10c inactivation causes high DIMBOA-Glc, low HDMBOA-Glc, and decreased aphid reproduction relative to near-isogenic lines that have a functional B×10c gene. These results confirm the importance of this locus in maize aphid resistance. The availability of B×10c near-isogenic lines will facilitate further research on the function of different benzoxazinoids and DIMBOA-Glc methyltransferase activity in maize defense against herbivores and pathogens.

Original languageEnglish
Article numbere26779
JournalPlant Signaling and Behavior
Volume8
Issue number10
DOIs
StatePublished - 25 Nov 2013
Externally publishedYes

Keywords

  • Benzoxazinoid
  • DIMBOA
  • HDMBOA
  • Rhopalosiphum maidis
  • Zea mays

ASJC Scopus subject areas

  • Plant Science

Fingerprint

Dive into the research topics of 'Near-isogenic lines for measuring phenotypic effects of DIMBOA-Glc methyltransferase activity in maize'. Together they form a unique fingerprint.

Cite this