Neat1 in Hematopoietic Stem Cells

Noam Fallik, Yael Bar-Lavan, Yariv Greenshpan, Oron Goldstein, Markus Grosch, Micha Drukker, Roi Gazit

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Hematopoietic Stem Cells (HSCs) generate blood and immune cells through a hierarchical process of differentiation. Genes that regulate this process are of great interest for understanding normal and also malignant hematopoiesis. Surprisingly, however, very little is known about long-non-coding RNAs (lncRNA) in HSCs. Neat1 is a lncRNA that plays a major role in the formation of sub-nuclear structures called paraspeckles, and was reported to regulate proliferation and differentiation in other cells types. We detected Neat1 expression using RNA-seq data and RT-qPCR in HSCs, progenitors and effector immune cells, by specific detection of its isoforms. Neat1 is highly expressed in stem and progenitor cells, yet it shows significant reduction in granulocytes. Microscopically, Neat1 is detected as sharp nuclear foci. Paraspeckle proteins NONO and PSPC1 are detected as aggregated nuclear foci in fresh primary hematopoietic cells, and in cultured cells. Induction of differentiation in vitro was found to enhance Neat1 expression. Taken together, our data demonstrate for the first time the expression of Neat1 and paraspeckles formation in HSCs and along hematopoiesis.

Original languageEnglish
Pages (from-to)109575-109586
Number of pages12
JournalOncotarget
Volume8
Issue number65
DOIs
StatePublished - 1 Jan 2017

Keywords

  • Hematopoiesis
  • Hematopoietic stem cells
  • Long non-coding RNAs
  • Neat1
  • Paraspeckles

ASJC Scopus subject areas

  • Oncology

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