TY - JOUR
T1 - Nematicidal activity of microbial pigment from Serratia marcescens
AU - Rahul, Suryawanshi
AU - Chandrashekhar, Patil
AU - Hemant, Borase
AU - Chandrakant, Narkhede
AU - Laxmikant, Shinde
AU - Satish, Patil
N1 - Funding Information:
We are thankful to Rajiv Gandhi Science and Technology Commission, Mumbai, Government of Maharashtra, India for financial assistance. We are also thankful to North Maharashtra Microbial Culture Collection Centre for providing microbial cultures.
PY - 2014/9/2
Y1 - 2014/9/2
N2 - Ineffectiveness of available nematicides and the high damage caused by plant-parasitic nematodes result in the urgent need to find some natural remedy for their control. Bioactivity of the pigment extracted from Serratia marcescens was screened for controlling nematodes at their juvenile stage. Test pigment was found effective against juvenile stages of Radopholus similis and Meloidogyne javanica at low concentrations (LC50 values, 83 and 79 g/mL, respectively) as compared with positive control of copper sulphate (LC50 values, 380 and 280 g/mL, respectively). The pigment also exhibited inhibition on nematode egg-hatching ability. Characterisation of extracted pigment with TLC, FTIR, HPLC, HPTLC and spectroscopic analysis confirmed the presence of prodigiosin as a bioactive metabolite. Considering the sensory mechanism of pathogen recognition by nematodes, the use of microbial secondary metabolites can be effective for nematode control rather than using whole organism.
AB - Ineffectiveness of available nematicides and the high damage caused by plant-parasitic nematodes result in the urgent need to find some natural remedy for their control. Bioactivity of the pigment extracted from Serratia marcescens was screened for controlling nematodes at their juvenile stage. Test pigment was found effective against juvenile stages of Radopholus similis and Meloidogyne javanica at low concentrations (LC50 values, 83 and 79 g/mL, respectively) as compared with positive control of copper sulphate (LC50 values, 380 and 280 g/mL, respectively). The pigment also exhibited inhibition on nematode egg-hatching ability. Characterisation of extracted pigment with TLC, FTIR, HPLC, HPTLC and spectroscopic analysis confirmed the presence of prodigiosin as a bioactive metabolite. Considering the sensory mechanism of pathogen recognition by nematodes, the use of microbial secondary metabolites can be effective for nematode control rather than using whole organism.
KW - Meloidogyne javanica
KW - Radopholus similis
KW - Serratia
KW - nematode
KW - pigment
UR - https://www.scopus.com/pages/publications/84905388395
U2 - 10.1080/14786419.2014.904310
DO - 10.1080/14786419.2014.904310
M3 - Article
C2 - 24697144
AN - SCOPUS:84905388395
SN - 1478-6419
VL - 28
SP - 1399
EP - 1404
JO - Natural Product Research
JF - Natural Product Research
IS - 17
ER -