New Enzymatic Synthesis of 2′-Deoxynucleoside-2′, 2′-d2and the Determination of Sugar Ring Flexibility by Solid-State Deuterium NMR

Siddhartha Roy; Jack S. Cohen; Yukio Hiyama; Dennis A. Torchia

doi:10.1021/ja00267a043

New Enzymatic Synthesis of 2′-Deoxynucleoside-2′, 2′-d₂and the Determination of Sugar Ring Flexibility by Solid-State Deuterium NMR

Siddhartha Roy, Jack S. Cohen, Yukio Hiyama, Dennis A. Torchia

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

An enzymatic synthesis of nucleosides selectively deuterated in the deoxyribose ring is described. The method is illustrated with the synthesis of 2′-deoxyguanosine-2′, 2′-d₂ and thymidine-2′, 2‘-d₂. The availability of such specifically labeled nucleosides enables the motional characteristics of the deoxyribose ring to be investigated with deuterium NMR spectroscopy in solution and the solid state.

Original language	English
Pages (from-to)	1675-1678
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	108
Issue number	7
DOIs	https://doi.org/10.1021/ja00267a043
State	Published - 1 Jan 1986
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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abstract = "An enzymatic synthesis of nucleosides selectively deuterated in the deoxyribose ring is described. The method is illustrated with the synthesis of 2′-deoxyguanosine-2′, 2′-d2 and thymidine-2′, 2{\textquoteleft}-d2. The availability of such specifically labeled nucleosides enables the motional characteristics of the deoxyribose ring to be investigated with deuterium NMR spectroscopy in solution and the solid state.",

author = "Siddhartha Roy and Cohen, {Jack S.} and Yukio Hiyama and Torchia, {Dennis A.}",

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AU - Hiyama, Yukio

AU - Torchia, Dennis A.

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Y1 - 1986/1/1

N2 - An enzymatic synthesis of nucleosides selectively deuterated in the deoxyribose ring is described. The method is illustrated with the synthesis of 2′-deoxyguanosine-2′, 2′-d2 and thymidine-2′, 2‘-d2. The availability of such specifically labeled nucleosides enables the motional characteristics of the deoxyribose ring to be investigated with deuterium NMR spectroscopy in solution and the solid state.

AB - An enzymatic synthesis of nucleosides selectively deuterated in the deoxyribose ring is described. The method is illustrated with the synthesis of 2′-deoxyguanosine-2′, 2′-d2 and thymidine-2′, 2‘-d2. The availability of such specifically labeled nucleosides enables the motional characteristics of the deoxyribose ring to be investigated with deuterium NMR spectroscopy in solution and the solid state.

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New Enzymatic Synthesis of 2′-Deoxynucleoside-2′, 2′-d₂and the Determination of Sugar Ring Flexibility by Solid-State Deuterium NMR

Abstract

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