New nanocomposites for SERS studies of living cells and mitochondria

A. S. Sarycheva, N. A. Brazhe, A. A. Baizhumanov, E. I. Nikelshparg, A. A. Semenova, A. V. Garshev, A. E. Baranchikov, V. K. Ivanov, G. V. Maksimov, O. Sosnovtseva, E. A. Goodilin

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


A great enhancement in Raman scattering (SERS) from heme-containing submembrane biomolecules inside intact erythrocytes and functional mitochondria is demonstrated for the first time using silver-silica beads prepared using a new method involving aerosol pyrolysis with aqueous diamminesilver(i) hydroxide as a unique source of plasmonic nanoparticles for SiO2 microspheres. The recorded SERS spectra reveal a set of characteristic peaks at 750, 1127, 1170, 1371, 1565, 1585 and 1638 cm-1, resulting from the normal group vibrations of the pyrrole rings, methine bridges and side radicals in the heme molecules. The SERS spectra of functional mitochondria are sensitive to the activity of the mitochondrial electron transport chain, thus making the method a novel label-free approach to monitor the redox state and conformation of cytochromes in their natural cell environment. The developed nanocomposites are highly suitable for the analysis of biological objects due to their robust synthesis and superior spatial and temporal signal reproducibility, which was preserved for a period of at least one year.

Original languageEnglish
Pages (from-to)539-546
Number of pages8
JournalJournal of Materials Chemistry B
Issue number3
StatePublished - 1 Jan 2016
Externally publishedYes

ASJC Scopus subject areas

  • General Chemistry
  • Biomedical Engineering
  • General Materials Science


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