TY - JOUR
T1 - New, sensitive thin-layer chromatographic-high-performance liquid chromatographic method for detection of trichothecene mycotoxins
AU - Yagen, Boris
AU - Sintov, Amnon
AU - Bialer, Meir
N1 - Funding Information:
This work was abstractedfr om the doctoral dissertationo f Mr. A. Sintov as partial fulfilmento f the Ph.D. requirementso f the Hebrew University of Jerusalem. This work was supportedb y a grant from the Office of the Chief Scientisto f the Israel Ministry of Health and the Israel National Council for Researcha nd Development.
PY - 1986/1/1
Y1 - 1986/1/1
N2 - Diphenylindenone sulphonyl (Dis) esters of trichothecene mycotoxins when sprayed with sodium methoxide showed fluorescent spots on a thin layer of silica gel when viewed under long-wavelength UV light. The detection limit for trichothecene esters in thin-layer chromatography (TLC) was 20-25 ng per spot for T-2 toxin, HT-2 toxin, diacetoxyscirpenol, T-2 triol, T-2 tetraol and iso-HT-2 toxin. A quantitative high-performance liquid chromatographic (HPLC) analysis of Dis-trichothecene esters was also developed using UV detection at 278 nm. The detection limit for the above esters varied between 30 and 50 ng per injection. This sensitive TLC-HPLC method is very useful for in vivo pharmacokinetic analyses of trichothecenes.
AB - Diphenylindenone sulphonyl (Dis) esters of trichothecene mycotoxins when sprayed with sodium methoxide showed fluorescent spots on a thin layer of silica gel when viewed under long-wavelength UV light. The detection limit for trichothecene esters in thin-layer chromatography (TLC) was 20-25 ng per spot for T-2 toxin, HT-2 toxin, diacetoxyscirpenol, T-2 triol, T-2 tetraol and iso-HT-2 toxin. A quantitative high-performance liquid chromatographic (HPLC) analysis of Dis-trichothecene esters was also developed using UV detection at 278 nm. The detection limit for the above esters varied between 30 and 50 ng per injection. This sensitive TLC-HPLC method is very useful for in vivo pharmacokinetic analyses of trichothecenes.
UR - http://www.scopus.com/inward/record.url?scp=0022492846&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(00)91479-1
DO - 10.1016/S0021-9673(00)91479-1
M3 - Article
AN - SCOPUS:0022492846
SN - 0021-9673
VL - 356
SP - 195
EP - 201
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - C
ER -